PPARG antibody (Internal Region, pSer273)
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- Target See all PPARG Antibodies
- PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))
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Binding Specificity
- Internal Region, pSer273
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This PPARG antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA
- Cross-Reactivity (Details)
- A BLAST analysis was used to suggest cross-reactivity with rat, mouse, primate, human, dog, bovine, and pig based on 100% sequence homology. Cross-reactivity with PPAR gamma pS273 from other sources has not been determined.
- Purification
- Anti-PPAR gamma pS273 was affinity purified from monospecific antiserum by immunoaffinity chromatography and is directed against the phosphorylated form of human PPAR gamma at the S273 residue.
- Immunogen
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Immunogen: Anti-PPAR gamma pS273 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the internal region of human PPARG protein.
Immunogen Type: Peptide
- Isotype
- IgG
- Top Product
- Discover our top product PPARG Primary Antibody
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- Application Notes
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Application Note: Anti-PPAR antibody is useful for ELISA, Immunohistochemistry, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~57 kDa corresponding to the appropriate cell lysate or extract.
Western Blot Dilution: 1 μg/mL
ELISA Dilution: 1: 10,000
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.85 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))
- Alternative Name
- PPAR gamma (PPARG Products)
- Synonyms
- PPAR gamma antibody, PPARg2 antibody, PPARG antibody, Nr1c3 antibody, PPAR-gamma antibody, PPAR-gamma2 antibody, PPARgamma antibody, PPARgamma2 antibody, NR1C3 antibody, CIMT1 antibody, GLM1 antibody, PPARG1 antibody, PPARG2 antibody, xPPAR-gamma antibody, PPAR-GAMMA antibody, PPARGAMMA antibody, peroxisome proliferator-activated receptor gamma antibody, peroxisome proliferator activated receptor gamma antibody, peroxisome proliferator activated receptor gamma L homeolog antibody, PPARG antibody, Pparg antibody, pparg.L antibody
- Background
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Synonyms: Peroxisome proliferator-activated receptor gamma, Nuclear receptor subfamily 1 group C member 3, NR1C3
Background: PPAR has a receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. The receptor, when activated by ligand, binds to a promotor element in the gene for acyl-CoA oxidase and activates its transcription. In this way it controls the peroxisomal beta-oxidation pathway of fatty acids. It plays a key role in regulating adipocyte differentiation, gut homeostasis, and glucose homeostasis. Anti-PPAR gamma pS273 Antibody is ideal for researchers interested in Metabolism, Cardiovascular or Neuroscience research.
Gene Name: PPARG
- Pathways
- MAPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Endopeptidase Activity, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation
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