SLC2A2 antibody (C-Term)

Catalog No. ABIN5596790

Product Details anti-SLC2A2 Antibody

Target: SLC2A2 (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 2 (SLC2A2))

Binding Specificity: C-Term

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC2A2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), FLISA

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with Glut2 from mouse and rat based on a 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

Purification: This affinity-purified Glut-2 antibody is directed against Glut2 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogen:

Immunogen: This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminal domain of mouse Glut2 protein.

Immunogen Type: Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 4 μg/mL

Application Note: Anti-Glut-2 antibody has been tested for use in ELISA, immunohistochemistry, and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 57.1 kDa in size corresponding to Glut2 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract.

Western Blot Dilution: 1:500-1:1000

FLISA Dilution: 1

ELISA Dilution: 1:30,000-1:90:000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-SLC2A2 antibody (ABIN5596790)

Liang, Yee, Chien, Chen, Luo, Zou, Piao, Mifune, Chen, Calvert, King, Norheim, Abad, Krauss, Giacomini: "Organic cation transporter 1 (OCT1) modulates multiple cardiometabolic traits through effects on hepatic thiamine content." in: PLoS biology, Vol. 16, Issue 4, pp. e2002907, (2019) (PubMed).

Chhabra, Morgan, Tooke, Adams, Rahmouni, Low: "Reduced renal sympathetic nerve activity contributes to elevated glycosuria and improved glucose tolerance in hypothalamus-specific Pomc knockout mice." in: Molecular metabolism, Vol. 6, Issue 10, pp. 1274-1285, (2018) (PubMed).

Chhabra, Adams, Fagel, Lam, Qi, Rubinstein, Low: "Hypothalamic POMC Deficiency Improves Glucose Tolerance Despite Insulin Resistance by Increasing Glycosuria." in: Diabetes, Vol. 65, Issue 3, pp. 660-72, (2016) (PubMed).

Target Details for SLC2A2

Target: SLC2A2 (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 2 (SLC2A2))

Alternative Name: Glut2 (SLC2A2 Products)

Background:

Synonyms: Solute carrier family 2, facilitated glucose transporter member 2, Glucose transporter type 2, liver-GLUT-2, Slc2a2, Glut2 Antibody

Background: The Anti-Glut2 antibody was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). The glucose transporter GLUT2 is a transmembrane carrier protein that allows protein facilitated glucose movement across cell membranes. GLUT2 is expressed in the plasma membranes of the liver, intestine, renal tubular cells, pancreatic islet beta cells, as well as in the portal and hypothalamic areas. Due to its low affinity and high capacity, GLUT2 transports dietary sugars, glucose, galactose and fructose in high concentrations, displaying large bidirectional fluxes in and out of cells. In pancreatic beta cells, GLUT2 is essential for glucose-stimulated insulin secretion. GLUT2 expression is necessary for the physiological control of glucose-sensitive genes, and its inactivation in the liver leads to impaired glucose-stimulated insulin secretion. In the nervous system, GLUT2-dependent glucose sensing regulates feeding, thermoregulation and pancreatic islet cell mass and function, as well as sympathetic and parasympathetic activities. In humans, inactivating mutations in GLUT2 cause Fanconi-Bickel syndrome, which is characterized by hepatomegaly and kidney disease. Anti-Glut2 is ideal for researchers interested in studying glucose transport mediated by Glut2 protein in the fields of diabetes, obesity, metabolism, and neuroscience research.

Gene Name: Slc2a2

Gene ID: 20526

UniProt: P14246

Pathways: Warburg Effect