BLM antibody (Internal Region, pThr99)

Catalog No. ABIN5596805

Product Details anti-BLM Antibody

Target: BLM (Bloom Syndrome RecQ Like Helicase (BLM))

Binding Specificity: Internal Region, pThr99

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BLM antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with human based on 100% sequence homology. Cross-reactivity with BLM pT99 from other sources has not been determined.

Purification: Anti-BLM pT99 was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Immunogen:

Immunogen: Anti-BLM pT99 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the internal region of human Bloom syndrome protein.

Immunogen Type: Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-BLM pT99 antibody is useful for ELISA, Immunohistochemistry, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~159 kDa corresponding to the appropriate cell lysate or extract.

Western Blot Dilution: 1 μg/mL

ELISA Dilution: 1: 10,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.11 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for BLM

Target: BLM (Bloom Syndrome RecQ Like Helicase (BLM))

Alternative Name: BLM (BLM Products)

Synonyms: BS antibody, RECQ2 antibody, RECQL2 antibody, RECQL3 antibody, blm-A antibody, xBLM antibody, ORF1 antibody, Bloom syndrome RecQ like helicase antibody, Bloom syndrome RecQ like helicase S homeolog antibody, Bloom syndrome, RecQ like helicase antibody, BLM antibody, blm.S antibody, Blm antibody

Background:

Synonyms: DNA helicase, RecQ-like type 2, RECQ2, RECQL3, Bloom syndrome protein

Background: BLM pT99 product is a member of the RecQ helicase protein family. The protein contains 1417 amino acids, including one ATP binding site, one DEAH box, and two putative nuclear localization signals, as well as phosphorylation sites. BLS protein is known to associate and be phosphorylated by the ATR (ataxia telangiectasia, rad3+) protein, in response to genetic stress. The Bloom's syndrome gene product is thought to play a role in the etiology of two major human health problems, each of which is a very common complication of Bloom's syndrome, namely cancer and diabetes. Anti-BLM pT99 antibody is ideal for researchers interested in Epigenetics and Nuclear Signaling or DNA Damage & Repair research.

Gene Name: BLM

Pathways: DNA Damage Repair