MST1R antibody (Internal Region, pTyr1353)

Catalog No. ABIN5596949

Product Details anti-MST1R Antibody

Target: MST1R (Macrophage Stimulating 1 Receptor (C-Met-Related tyrosine Kinase) (MST1R))

Binding Specificity: Internal Region, pTyr1353

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MST1R antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Cross-Reactivity (Details): Cross-reactivity against RON from other species may occur but has not yet been tested.

Purification: Anti-RON pY1353 was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorptions against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. This antibody is specific for human RON protein phosphorylated at Y1353.

Immunogen:

Immunogen: Anti-RON pY1353 Antibody was produced in rabbits by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y1353 of human RON protein.

Immunogen Type: Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-RONpY1353 Antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band at ~ 152.3 kDa corresponding to full length protein by western blotting in the appropriate cell lysate or extract. This phospho-specific polyclonal antibody reacts with human RON pY1353 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.

Western Blot Dilution: 1:500-1:2000

ELISA Dilution: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for MST1R

Target: MST1R (Macrophage Stimulating 1 Receptor (C-Met-Related tyrosine Kinase) (MST1R))

Alternative Name: RON (MST1R Products)

Synonyms: CD136 antibody, CDw136 antibody, PTK8 antibody, RON antibody, Fv-2 antibody, Fv2 antibody, Ron antibody, STK antibody, Cdw136 antibody, Ptk8 antibody, Stk antibody, macrophage stimulating 1 receptor antibody, macrophage stimulating 1 receptor (c-met-related tyrosine kinase) antibody, MST1R antibody, Mst1r antibody

Background:

Synonyms: Macrophage-stimulating protein receptor, p185-Ron, PTK8, RON, Protein-tyrosine kinase 8, c-met-related tyrosine kinase

Background: RON pY1353 antibody detects phosphorylated RON. RON is a member of the protein kinase superfamily. Protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. RON is a heterodimer composed of an α chain and a β chain. The α chain is completely extracellular, whereas the β chain traverses the cell membrane and contains the intracellular tyrosine kinase and regulatory elements. Anti-RON Antibody is ideal for investigators involved in Cell Signaling, Cancer, Neuroscience and Signal Transduction research.

Gene Name: MST1R

Gene ID: 4486

UniProt: Q04912

Pathways: RTK Signaling