Goat anti-Ferret IgG (Heavy Chain) Antibody (Biotin) - Preadsorbed

Catalog No. ABIN5608045

Product Details

Target: IgG

Binding Specificity: Heavy Chain

Reactivity: Ferret

Host: Goat

Clonality: Polyclonal

Conjugate: Biotin

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Characteristics: Anti-FERRET IgG (gamma chain) (GOAT) Antibody generated in goat detects specifically Ferret IgG gamma chain. Ferret antibody is ideal for investigators involved in Serum Protein Component research.

Purification: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Ferret IgG gamma heavy chain

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Anti-FERRET IgG (gamma chain) (GOAT) Antibody is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry requiring extremely low background levels, lot-to-lot consistency, high titer and specificity.

ELISA Dilution: 1:20,000 - 1:100,000

Western Blot Dilution: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References

Jackson-Thompson, Kim, Jaiswal, Scott, Jones, Morris, Fite, Laurie, Hoy, Dardzinski, Mitre: "Brugia malayi infection in ferrets - A small mammal model of lymphatic filariasis." in: PLoS neglected tropical diseases, Vol. 12, Issue 3, pp. e0006334, (2018) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background:

Synonyms: goat anti-Ferret IgG Antibody Biotin Conjugation, goat anti-Ferret IgG Biotin Conjugated Antibody

Background: Anti-Ferret IgG Biotin antibody generated in goat detects ferret IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.