Goat anti-Ferret IgM (Heavy & Light Chain) Antibody (Alkaline Phosphatase (AP)) - Preadsorbed
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- Target See all IgM products
- IgM
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Binding Specificity
- Heavy & Light Chain
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Reactivity
- Ferret
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- Alkaline Phosphatase (AP)
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Application
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Purification
- Preadsorption: Solid phase absorption
- Immunogen
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Immunogen: Ferret IgM whole molecule
- Isotype
- IgG
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- Application Notes
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Immunohistochemistry Dilution: 1:200 - 1:1,000
Application Note: Anti-Ferret IgM Alk Phos conjugate is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.
ELISA Dilution: 1:1,000 - 1:2,500
Western Blot Dilution: 1:500 - 1:2,500
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.1 mg/mL
- Buffer
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Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
- Expiry Date
- 12 months
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- Target
- IgM
- Abstract
- IgM Products
- Target Type
- Antibody
- Background
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Synonyms: goat anti-Ferret IgM mu chain Antibody alkaline phosphatase conjugation, goat anti-ferret IgM mu alk phos conjugated antibody
Background: Anti-Ferret IgM antibody specifically detects ferret IgM. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. Anti-Ferret IgM antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.
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