Goat anti-Ferret IgG (Heavy Chain) Antibody (HRP) - Preadsorbed
-
- Target See all IgG products
- IgG
-
Binding Specificity
- Heavy Chain
-
Reactivity
- Ferret
-
Host
- Goat
-
Clonality
- Polyclonal
-
Conjugate
- HRP
-
Application
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Cross-Reactivity
- Ferret
- Characteristics
- Anti-Ferret IgG (gamma chain) peroxidase conjugated antibody generated in goat detects specifically ferret IgG (gamma chain). This secondary peroxidase conjugated antibody anti-Ferret is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
- Purification
- Preadsorption: Solid phase absorption
- Immunogen
-
Immunogen: Anti-Ferret IgG (gamma chain) was produced by repeated immunization with ferret IgG gamma heavy chain in goat.
Immunogen Type: Native Protein
- Isotype
- IgG
-
-
- Application Notes
-
Immunohistochemistry Dilution: 1:500 - 1:2,500
Application Note: Antibody Anti-Ferret IgG (gamma chain) peroxidase conjugated is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
ELISA Dilution: 1:10,000 - 1:50,000
Western Blot Dilution: 1:1,000 - 1:5,000
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
-
Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 1.0 mg/mL
- Buffer
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Thimerosal
- Preservative
- Thimerosal (Merthiolate)
- Precaution of Use
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- RT,4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
-
- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
- Background
-
Synonyms: goat anti-Ferret IgG (gamma chain) Antibody peroxidase Conjugation, goat anti-Ferret IgG gamma HRP Conjugated Antibody
Background: Anti-Ferret IgG Peroxidase Antibody generated in goat detects ferret IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.
-
