MLKL antibody (pSer358)

Catalog No. ABIN5610033

Product Details anti-MLKL Antibody

Target: MLKL (Mixed Lineage Kinase Domain-Like (MLKL))

Binding Specificity: pSer358

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MLKL antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: MLKL is a protein encoded by the MLKL gene which is approximately 54,4 kDa. MLKL is localised to the cell membrane and cytoplasm. It is involved in the dimerization of procaspase-8, the TNF signalling pathway, apoptosis and autophagy. This protein falls under the protein kinase superfamily. It plays a critical role in tumour necrosis factor-induced necroptosis which is a programmed cell death process. It does this via interaction with the receptor-interacting protein 3 which is a key signalling molecule in necroptosis pathway. MLKL is expressed in the heart, intestine and lung. STJ97776 was developed from clone 6F8 and was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen MLKL. This primary antibody specifically binds to endogenous MLKL protein which only binds about S358 when S358 is phosphorylated.

Specificity: Phospho-MLKL (S358) Mouse Monoclonal Antibody (6F8) detects endogenous levels of MLKL

Characteristics: Mouse Monoclonal to phospho-MLKL (S358).

Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Immunogen: synthetic peptide derived from phospho-MLKL (S358)

Clone: 6F8

Isotype: IgG1

Application Details

Application Notes: IHC 1:100-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Store at -20°C, and avoid repeat freeze-thaw cycles.

Target Details for MLKL

Target: MLKL (Mixed Lineage Kinase Domain-Like (MLKL))

Alternative Name: MLKL (MLKL Products)

Synonyms: MLKL antibody, 9130019I15Rik antibody, mixed lineage kinase domain like pseudokinase antibody, mixed lineage kinase domain-like antibody, MLKL antibody, mlkl antibody, Mlkl antibody

Gene ID: 197259

UniProt: Q8NB16