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ASK1 antibody (Ser1033)

MAP3K5 Reactivity: Human WB, IHC (p), EIA Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN360294
  • Target See all ASK1 (MAP3K5) Antibodies
    ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))
    Binding Specificity
    • 26
    • 26
    • 18
    • 17
    • 16
    • 12
    • 12
    • 9
    • 7
    • 5
    • 5
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Ser1033
    Reactivity
    • 182
    • 100
    • 57
    • 18
    • 15
    • 8
    Human
    Host
    • 191
    • 8
    Rabbit
    Clonality
    • 171
    • 28
    Polyclonal
    Conjugate
    • 81
    • 12
    • 12
    • 9
    • 8
    • 8
    • 8
    • 8
    • 8
    • 8
    • 6
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 1
    This ASK1 antibody is un-conjugated
    Application
    • 158
    • 58
    • 39
    • 39
    • 38
    • 29
    • 29
    • 20
    • 11
    • 11
    • 6
    • 4
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
    Specificity
    This antibody reacts to ASK1 (MEKK5/MAP3K5).
    Purification
    Affinity chromatography on Protein A
    Immunogen
    KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S83 of human MAP3K5.
    Isotype
    Ig Fraction
    Top Product
    Discover our top product MAP3K5 Primary Antibody
  • Application Notes
    ELISA: 1/1,000. Western blotting: 1/50 - 1/100. Immunohistochemistry: 1/10 - 1/50.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.25 mg/mL
    Buffer
    PBS with 0.09 % (W/V) sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
  • Target
    ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))
    Alternative Name
    MEKK5 / ASK1 (MAP3K5 Products)
    Synonyms
    ASK1 antibody, MAPKKK5 antibody, MEKK5 antibody, mitogen-activated protein kinase kinase kinase 5 antibody, MUD21.11 antibody, MUD21_11 antibody, 7420452D20Rik antibody, ASK antibody, Mekk5 antibody, Ask1 antibody, RGD1306565 antibody, mitogen-activated protein kinase kinase kinase 5 antibody, MAP3K5 antibody, MAPKKK5 antibody, Map3k5 antibody, map3k5 antibody
    Background
    Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells, MAPKKK5 does not activate MAPK/ERK.Synonyms: Apoptosis signal-regulating kinase 1, MAP3K5, MAPK/ERK kinase kinase 5, MAPKKK5, Mitogen-activated protein kinase kinase kinase 5
    Gene ID
    4217, 9606
    UniProt
    Q99683
    Pathways
    MAPK Signaling, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response
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