RANKL antibody (AA 74-308)

Catalog No. ABIN5611144

Product Details anti-RANKL Antibody

Target: RANKL (TNFSF11) (Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))

Binding Specificity: AA 74-308

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This RANKL antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC), Neutralization (Neut)

Purification: purified

Immunogen: Purified recombinant fragment of human TNFSF11 (AA: 74-308) expressed in E. coli.

Clone: 3H8A7

Isotype: IgG1

Application Details

Application Notes: ELISA: 1:10000, WB: 1:500 - 1:2000, ICC: N/A, FCM: 1:200 - 1:400, IHC: 1:200 - 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified antibody in PBS with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: 4°C, -20°C for long term storage

Target Details for RANKL

Target: RANKL (TNFSF11) (Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))

Alternative Name: TNFSF11 (TNFSF11 Products)

Background:

Description: This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found.

Aliases: CD254, ODF, OPGL, sOdf, OPTB2, RANKL, TNLG6B, TRANCE, hRANKL2

Molecular Weight: 35.5 kDa

Gene ID: 8600

HGNC: 8600

Pathways: NF-kappaB Signaling