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SEMA4D/CD100 antibody (AA 590-734)

Name: SEMA4D/CD100 antibody (AA 590-734)
Brand: antibodies-online
SKU: ABIN5611305
Price: 511.5 USD
Availability: InStock

SEMA4D Reactivity: Human WB, FACS, IHC, ELISA, ICC, Neut Host: Mouse Monoclonal 5C5B10 unconjugated

Western blot analysis using CD100 mAb against HEK293 (1) and CD100 (AA: extra 590-734)-hIgGFc transfected HEK293 (2) cell lysate.

Flow cytometric analysis of Ramos cells using CD100 mouse mAb (green) and negative control (red).

FACS

Black line: Control Antigen (100 ng),Purple line: Antigen (10 ng), Blue line: Antigen (50 ng), Red line:Antigen (100 ng)

ELISA

Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using CD100 mouse mAb with DAB staining.

IHC

Western blot analysis using CD100 mAb against human CD100 (AA: extra 590-734) recombinant protein.

Flow cytometric analysis of K562 cells using CD100 mouse mAb (green) and negative control (red).

FACS

6 images

Catalog No. ABIN5611305

Datasheet as PDF

Target See all SEMA4D/CD100 (SEMA4D) Antibodies

SEMA4D/CD100 (SEMA4D) (Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D))
Binding Specificity
5

4

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1
AA 590-734
Reactivity
64

19

8

7

4

4

3

3

3

3

2

1

1

1

1
Human
Host
34

33

4

1
Mouse
Compatible Secondaries
Clonality
39

33
Monoclonal
Conjugate
49

6

5

3

2

1

1

1

1

1

1

1
This SEMA4D/CD100 antibody is un-conjugated
Application
37

31

19

15

14

9

5

5

2

2

1
Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Neutralization (Neut)

Purification

purified

Immunogen

Purified recombinant fragment of human CD100 (AA: extra 590-734) expressed in E. coli.

Clone

5C5B10

Isotype

IgG1

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SEMA4D Reactivity: Human WB, FACS, IHC, ELISA, ICC, Neut Host: Mouse Monoclonal 5H6E3 unconjugated

anti-Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D) (AA 115-224) antibody
SEMA4D Reactivity: Human WB, ELISA Host: Mouse Monoclonal 3B4 unconjugated

anti-Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D) antibody (FITC)
SEMA4D Reactivity: Human FACS Host: Mouse Monoclonal 133-1C6 FITC

anti-Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D) (AA 502-636) antibody
SEMA4D Reactivity: Mouse WB, IP, IHC, ICC Host: Rabbit Polyclonal unconjugated

anti-Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D) (AA 145-494) antibody
SEMA4D Reactivity: Rat WB, IP, IHC, ICC Host: Rabbit Polyclonal unconjugated

anti-Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D) (C-Term) antibody
SEMA4D Reactivity: Human WB, IHC, ELISA Host: Rabbit Polyclonal unconjugated

anti-Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D) (AA 80-93), (Extracellular), (N-Term) antibody
SEMA4D Reactivity: Human, Mouse, Rat WB, FACS Host: Rabbit Polyclonal unconjugated

anti-Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D) (N-Term) antibody
SEMA4D Reactivity: Human WB, ELISA Host: Rabbit Polyclonal unconjugated

anti-Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D) (AA 502-636) antibody
SEMA4D Reactivity: Human WB, IP, IHC, ICC Host: Rabbit Polyclonal unconjugated

anti-Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D) (AA 756-862) antibody
SEMA4D Reactivity: Human ELISA, IF Host: Rabbit Polyclonal unconjugated

Application Notes

ELISA: 1:10000, WB: 1:500 - 1:2000, ICC: N/A, FCM: 1:200 - 1:400, IHC: 1:200 - 1:1000

Restrictions

For Research Use only
Format

Liquid

Buffer

Purified antibody in PBS with 0.05 % sodium azide

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C/-20 °C

Storage Comment

4°C, -20°C for long term storage
Target

SEMA4D/CD100 (SEMA4D) (Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D))

Alternative Name

CD100 (SEMA4D Products)

Synonyms

C9orf164 antibody, CD100 antibody, M-sema-G antibody, SEMAJ antibody, coll-4 antibody, Semacl2 antibody, Semaj antibody, Semcl2 antibody, COLL-4 antibody, semaphorin 4D antibody, sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D antibody, Sema4d antibody, SEMA4D antibody

Background

Description: SEMA4D (Semaphorin 4D) is a Protein Coding gene. Diseases associated with SEMA4D include Hemorrhagic Fever With Renal Syndrome. Among its related pathways are Guidance Cues and Growth Cone Motility and Developmental Biology. GO annotations related to this gene include receptor binding and transmembrane signaling receptor activity. An important paralog of this gene is SEMA4B.
Aliases: SEMA4D, SEMAJ, coll-4, C9orf164, M-sema-G

Molecular Weight

96.2 kDa

Gene ID

10507

HGNC

10507

Pathways

Regulation of Cell Size