YAP1 antibody (AA 53-161)

Catalog No. ABIN564526

Product Details anti-YAP1 Antibody

Target: YAP1 (Yes-Associated Protein 1 (YAP1))

Binding Specificity: AA 53-161

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This YAP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Mouse monoclonal antibody raised against a partial recombinant YAP1.

Sequence: QIVHVRGDSE TDLEALFNAV MNPKTANVPQ TVPMRLRKLP DSFFKPPEPK SHSRQASTDA GTAGALTPQH VRAHSSPASL QLGAVSPGTL TPTGVVSGPA ATPTAQHLR

Cross-Reactivity: Human, Mouse

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: YAP1 (NP_006097, 53 a.a. ~ 161 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone: 2F12

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-YAP1 antibody (ABIN564526)

Toyooka, Oka, Fujimori: "Early preimplantation cells expressing Cdx2 exhibit plasticity of specification to TE and ICM lineages through positional changes." in: Developmental biology, Vol. 411, Issue 1, pp. 50-60, (2016) (PubMed).

Abbassi, Malki, Cockburn, Macaulay, Robert, Rossant, Clarke: "Multiple Mechanisms Cooperate to Constitutively Exclude the Transcriptional Co-Activator YAP from the Nucleus During Murine Oogenesis." in: Biology of reproduction, Vol. 94, Issue 5, pp. 102, (2016) (PubMed).

Junemann, Birgin, Erdmann, Schröder, Just, Gerhard, Pich: "Toxin A of the nosocomial pathogen Clostridium difficile induces primary effects in the proteome of HEp-2 cells." in: Proteomics. Clinical applications, Vol. 11, Issue 3-4, (2016) (PubMed).

Yang, Zhang, Chen, Chen, Dong: "Oncoprotein YAP regulates the spindle checkpoint activation in a mitotic phosphorylation-dependent manner through up-regulation of BubR1." in: The Journal of biological chemistry, Vol. 290, Issue 10, pp. 6191-202, (2015) (PubMed).

Shao, Zhai, Del Re, Sciarretta, Yabuta, Nojima, Lim, Pan, Sadoshima: "A functional interaction between Hippo-YAP signalling and FoxO1 mediates the oxidative stress response." in: Nature communications, Vol. 5, pp. 3315, (2014) (PubMed).

Adler, Heller, Bringman, Ranahan, Cocklin, Goebl, Oh, Lim, Ingham, Wells: "Amot130 adapts atrophin-1 interacting protein 4 to inhibit yes-associated protein signaling and cell growth." in: The Journal of biological chemistry, Vol. 288, Issue 21, pp. 15181-93, (2013) (PubMed).

Das, Tanigawa, Karner, Xin, Lum, Chen, Olson, Perantoni, Carroll: "Stromal-epithelial crosstalk regulates kidney progenitor cell differentiation." in: Nature cell biology, Vol. 15, Issue 9, pp. 1035-44, (2013) (PubMed).

Yang, Zhang, Liu, Chong, Ding, Chen, Dong: "CDK1 phosphorylation of YAP promotes mitotic defects and cell motility and is essential for neoplastic transformation." in: Cancer research, Vol. 73, Issue 22, pp. 6722-33, (2013) (PubMed).

Adler, Johnson, Heller, Bringman, Ranahan, Conwell, Sun, Hudmon, Wells: "Serum deprivation inhibits the transcriptional co-activator YAP and cell growth via phosphorylation of the 130-kDa isoform of Angiomotin by the LATS1/2 protein kinases." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 43, pp. 17368-73, (2013) (PubMed).

Skouloudaki, Walz: "YAP1 recruits c-Abl to protect angiomotin-like 1 from Nedd4-mediated degradation." in: PLoS ONE, Vol. 7, Issue 4, pp. e35735, (2012) (PubMed).

Hirate, Cockburn, Rossant, Sasaki: "Tead4 is constitutively nuclear, while nuclear vs. cytoplasmic Yap distribution is regulated in preimplantation mouse embryos." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 50, pp. E3389-90; author reply E3391-2, (2012) (PubMed).

Murakami, Mizuno, Taniguchi, Fujii, Ishiguro, Fukui, Akatsuka, Horio, Hida, Kondo, Toyokuni, Osada, Sekido: "LATS2 is a tumor suppressor gene of malignant mesothelioma." in: Cancer research, Vol. 71, Issue 3, pp. 873-83, (2011) (PubMed).

Leivonen, Rokka, Ostling, Kohonen, Corthals, Kallioniemi, Perälä: "Identification of miR-193b targets in breast cancer cells and systems biological analysis of their functional impact." in: Molecular & cellular proteomics : MCP, Vol. 10, Issue 7, pp. M110.005322, (2011) (PubMed).

Sekido: "Inactivation of Merlin in malignant mesothelioma cells and the Hippo signaling cascade dysregulation." in: Pathology international, Vol. 61, Issue 6, pp. 331-44, (2011) (PubMed).

Wada, Itoga, Okano, Yonemura, Sasaki: "Hippo pathway regulation by cell morphology and stress fibers." in: Development (Cambridge, England), Vol. 138, Issue 18, pp. 3907-14, (2011) (PubMed).

Tomlinson, Gudmundsdottir, Luong, Leung, Knebel, Basu: "JNK phosphorylates Yes-associated protein (YAP) to regulate apoptosis." in: Cell death & disease, Vol. 1, pp. e29, (2011) (PubMed).

Lau, Murray, Houshmandi, Xu, Gutmann, Yu: "Merlin is a potent inhibitor of glioma growth." in: Cancer research, Vol. 68, Issue 14, pp. 5733-42, (2008) (PubMed).

Target Details for YAP1

Target: YAP1 (Yes-Associated Protein 1 (YAP1))

Alternative Name: YAP1 (YAP1 Products)

Synonyms: YAP antibody, YAP2 antibody, YAP65 antibody, YKI antibody, xyap antibody, yap antibody, yap2 antibody, yap65 antibody, yki antibody, AI325207 antibody, Yap antibody, Yap65 antibody, Yki antibody, Yorkie antibody, cb194 antibody, sb:cb194 antibody, si:ch211-181p1.5 antibody, si:dkey-3b8.3 antibody, wu:fc18c04 antibody, zgc:158380 antibody, Yes associated protein 1 antibody, Yes associated protein 1 S homeolog antibody, yes-associated protein 1 antibody, Yes-associated protein 1 antibody, YAP1 antibody, yap1.S antibody, Yap1 antibody, yap1 antibody

Background: Full Gene Name: Yes-associated protein 1, 65 kDa
Synonyms: YAP,YAP2,YAP65,YKI

Gene ID: 10413

NCBI Accession: NM_006106

Pathways: MAPK Signaling, Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha