ECT2 antibody (AA 200-460)

Catalog No. ABIN5647111

Product Details anti-ECT2 Antibody

Target: ECT2 (Epithelial Cell Transforming Sequence 2 Oncogene (ECT2))

Binding Specificity: AA 200-460

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ECT2 antibody is un-conjugated

Application: Western Blotting (WB)

Purification: Purified

Immunogen: A partial recombinant protein corresponding to amino acids 200-460 from the human protein was used as the immunogen for the ECT2 antibody.

Clone: 1691CT516-3-3

Isotype: IgG1 kappa

Application Details

Application Notes: Western blot: 1:500-1000

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the ECT2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for ECT2

Target: ECT2 (Epithelial Cell Transforming Sequence 2 Oncogene (ECT2))

Alternative Name: ECT2 (ECT2 Products)

Synonyms: ECT2 antibody, arhgef31 antibody, xect2 antibody, AI528536 antibody, mKIAA4037 antibody, ARHGEF31 antibody, ect2 antibody, epithelial cell transforming 2 antibody, ect2 oncogene antibody, epithelial cell transforming 2 S homeolog antibody, ECT2 antibody, ect2 antibody, Ect2 antibody, ect2.S antibody

Background: Epithelial cell-transforming sequence 2 oncogene is a guanine nucleotide exchange factor (GEF) that catalyzes the exchange of GDP for GTP. Promotes guanine nucleotide exchange on the Rho family members of small GTPases, like RHOA, RHOC, RAC1 and CDC42. Required for signal transduction pathways involved in the regulation of cytokinesis. Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Regulates the translocation of RHOA from the central spindle to the equatorial region. Plays a role in the control of mitotic spindle assembly, regulates the activation of CDC42 in metaphase for the process of spindle fibers attachment to kinetochores before chromosome congression. Involved in the regulation of epithelial cell polarity, participates in the formation of epithelial tight junctions in a polarity complex PARD3-PARD6-protein kinase PRKCQ-dependent manner. Plays a role in the regulation of neurite outgrowth. Inhibits phenobarbital (PB)- induced NR1I3 nuclear translocation. Stimulates the activity of RAC1 through its association with the oncogenic PARD6A-PRKCI complex in cancer cells, thereby acting to coordinately drive tumor cell proliferation and invasion. Also stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death.

Gene ID: 1894

UniProt: Q9H8V3

Pathways: Neurotrophin Signaling Pathway, Cell-Cell Junction Organization