ELANE antibody

Catalog No. ABIN5647773

Product Details anti-ELANE Antibody

Target: ELANE (Elastase, Neutrophil Expressed (ELANE))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ELANE antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Antigen affinity purified

Immunogen: Amino acids S27-N265 from the mouse protein were used as the immunogen for the Neutrophil Elastase antibody.

Isotype: IgG

Application Details

Application Notes: Western blot: 0.5-1 μg/mL,IHC (FFPE): 1-2 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the Neutrophil Elastase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for ELANE

Target: ELANE (Elastase, Neutrophil Expressed (ELANE))

Alternative Name: Neutrophil Elastase (ELANE Products)

Synonyms: ELA2 antibody, GE antibody, HLE antibody, HNE antibody, NE antibody, PMN-E antibody, SCN1 antibody, Ela2 antibody, F430011M15Rik antibody, ela2 antibody, ELANE antibody, elastase, neutrophil expressed antibody, proteinase 3 antibody, ELANE antibody, Elane antibody, prtn3 antibody

Background: Neutrophil elastase, also called Elane, is a serine protease of neutrophil and monocyte granules. Its key physiologic role is in innate host defense, but it can also participate in tissue remodeling and possesses secretagogue actions important to local inflammatory responses. Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode structurally similar proteins. The encoded preproprotein is proteolytically processed to generate the active protease. Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. This protein also degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is present in a gene cluster on chromosome 19.

UniProt: Q3UP87