NFATC1 antibody (AA 110-210) (HRP)

Catalog No. ABIN5651057

Product Details anti-NFATC1 Antibody (HRP)

Target: NFATC1 (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 1 (NFATC1))

Binding Specificity: AA 110-210

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFATC1 antibody is conjugated to HRP

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: Expressed in thymus, peripheral leukocytes as T-cells and spleen. Isoforms A are preferentially expressed in effector T-cells (thymus and peripheral leukocytes) whereas isoforms B and isoforms C are preferentially expressed in naive T-cells (spleen). Isoforms B are expressed in naive T-cells after first antigen exposure and isoforms A are expressed in effector T-cells after second antigen exposure. Isoforms IA are widely expressed but not detected in liver nor pancreas, neural expression is strongest in corpus callosum. Isoforms IB are expressed mostly in muscle, cerebellum, placenta and thymus, neural expression in fetal and adult brain, strongest in corpus callosum.,Detects 90 kDa, band at ~60 kDa is another isoform.

Cross-Reactivity: Human, Rat

Purification: Peptide Affinity Purified

Immunogen: Synthetic peptide of Human NFATC1 (110-210 aa), conjugated to Keyhole Limpet Haemocyanin (KLH).

Application Details

Application Notes:

• WB (1:1000)
• IHC (1:50)
• optimal dilutions for assays should be determined by the user.

Comment:

A 1:1000 dilution of ABIN5651057 was sufficient for detection of NFATC1 in 15 μg of rat brain cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for NFATC1

Target: NFATC1 (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 1 (NFATC1))

Alternative Name: NFATC1 (NFATC1 Products)

Synonyms: nf-atc antibody, nfat2 antibody, nfatc antibody, si:ch211-198c22.2 antibody, NFATC1 antibody, 2210017P03Rik antibody, AI449492 antibody, AV076380 antibody, NF-ATc antibody, NFAT2 antibody, NFATc antibody, Nfatcb antibody, NF-ATC antibody, NF-ATc1 antibody, NFAT antibody, NFATmac antibody, nuclear factor of activated T-cells 1 antibody, nuclear factor of activated T-cells 1 L homeolog antibody, nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1 antibody, nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 1 antibody, NFATC1 antibody, nfatc1.L antibody, nfatc1 antibody, Nfatc1 antibody

Gene ID: 4772

NCBI Accession: NP_001265598

UniProt: O95644

Pathways: RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway