HSPB6 antibody (AA 1-162)

Catalog No. ABIN5661998

Product Details anti-HSPB6 Antibody

Target: HSPB6 (Heat Shock Protein, alpha-Crystallin-Related, B6 (HSPB6))

Binding Specificity: AA 1-162

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This HSPB6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Monoclonal Antibody to Heat Shock Protein Beta 6 (HSPb6)

Sequence: MGHHHHHHSG SEF-MEIRVPVQPS WLRRASAPLP GFSTPGRLFD QRFGEGLLEA ELASLCPAAI APYYLRAPSV ALPTAQVPTD PGYFSVLLDV KHFSPEEISV KVVGDHVEVH ARHEERPDEH GFIAREFHRR YRLPPGVDPA AVTSALSPEG VLSIQATPAS AQASLPSPPA AK

Specificity: The antibody is a mouse monoclonal antibody raised against HSPb6. It has been selected for its ability to recognize HSPb6 in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Mouse

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Heat Shock Protein Beta 6 (HSPb6) corresdonding to Met1~Lys162 (Accession # P97541) with N-terminal His Tag

Clone: D4

Isotype: IgG2a kappa

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

1:500-2000 Immunohistochemistry: 5-20 μg/mL

1:50-200 Immunocytochemistry: 5-20 μg/mL

1:50-200 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for HSPB6

Target: HSPB6 (Heat Shock Protein, alpha-Crystallin-Related, B6 (HSPB6))

Alternative Name: Heat Shock Protein Beta 6 (HSPB6 Products)

Synonyms: Hsp20 antibody, p20 antibody, AA387366 antibody, Gm479 antibody, HSP20 antibody, heat shock protein family B (small) member 6 antibody, heat shock protein, alpha-crystallin-related, B6 antibody, heat shock protein family B (small) member 6 L homeolog antibody, heat shock protein, alpha-crystallin-related, b6 antibody, HSPB6 antibody, Hspb6 antibody, hspb6.L antibody, hspb6 antibody

Background: HSP20, Heat Shock Protein 20 kD, Heat Shock Protein B6, Alpha Crystallin Related, Heat shock 20 kDa-like protein p20