Insulin Receptor antibody (N-Term)

Catalog No. ABIN1513096

Product Details anti-Insulin Receptor Antibody

Target: Insulin Receptor (INSR)

Binding Specificity: N-Term

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Insulin Receptor antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purification: Affinity purification

Immunogen: N term -peptide of human INSR

Isotype: IgG

Application Details

Application Notes: Recommended dilutions: WB: 1:500-1:2000, ICC: 1:50-1:200, IP: 1:20-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C/-80 °C

Target Details for Insulin Receptor

Target: Insulin Receptor (INSR)

Alternative Name: INSR (INSR Products)

Synonyms: CD220 antibody, HHF5 antibody, 4932439J01Rik antibody, D630014A15Rik antibody, IR antibody, IR-A antibody, IR-B antibody, 18402 antibody, CG18402 antibody, DIHR antibody, DILR antibody, DIR antibody, DIRH antibody, DIRbeta antibody, DInR antibody, DInr antibody, Dir-a antibody, Dir-b antibody, Dmel\\CG18402 antibody, INR antibody, INS antibody, Inr antibody, Inr-alpha antibody, Inr-beta antibody, InsR antibody, dINR antibody, dIR antibody, dIRH antibody, dInR antibody, dInr antibody, dInsR antibody, dinr antibody, dir antibody, er10 antibody, inr antibody, insulin/insulin-like growth factor receptor antibody, l(3)05545 antibody, l(3)93Dj antibody, l(3)er10 antibody, lnR antibody, ir-A antibody, CTK-1 antibody, ir antibody, INSR antibody, NV14476 antibody, cd220 antibody, hhf5 antibody, insulin receptor antibody, Insulin-like receptor antibody, insulin receptor L homeolog antibody, INSR antibody, Insr antibody, InR antibody, LOC100122567 antibody, LOC100451802 antibody, insr.L antibody

Background: Type I insulin-like growth factor receptor (IGF-IR) is a transmembrane receptor tyrosine kinase that is widely expressed in many cell lines and cell types within fetal and postnatal tissues. Receptor autophosphorylation follows binding of the IGF-I and IGF-II ligands. Three tyrosine residues within the kinase domain (Tyr1131, Tyr1135, and Tyr1136) are the earliest major autophosphorylation sites. Phosphorylation of these three tyrosine residues is necessary for kinase activation. Insulin receptors (IRs) share significant structural and functional similarity with IGF-I receptors, including the presence of an equivalent tyrosine cluster (Tyr1146/1150/1151) within the kinase domain activation loop. Tyrosine autophosphorylation of IRs is one of the earliest cellular responses to insulin stimulation. Autophosphorylation begins with phosphorylation of Tyr1146 and either Tyr1150 or Tyr1151, while full kinase activation requires triple tyrosine phosphorylation. 1. Adams, T.E. et al. Cell. Mol. Life Sci. 57, 1050-1093. 2. Baserga, R. et al. Oncogene 19, 5574-5581. 3. Scheidegger, K.J. et al. J. Biol. Chem. 275, 38921-38928. 4. Hernandez-Sanchez, C. et al. J. Biol. Chem. 270, 29176-29181. 5. Lopaczynski, W. et al. Biochem. Biophys. Res. Commun. 279, 955-960. 6. Baserga, R. et al. Exp. Cell Res. 253, 1-6. 7. White, M.F. et al. J. Biol. Chem. 260, 9470-9478. 8. White, M.F. et al. J. Biol. Chem. 263, 2969-2980.  
Synonyms: INSR, CD220, HHF5 , Insulin Receptor beta

Gene ID: 3643

UniProt: P06213

Pathways: NF-kappaB Signaling, RTK Signaling, AMPK Signaling, Carbohydrate Homeostasis, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Growth Factor Binding, Negative Regulation of Transporter Activity