Recombinant F4/80 antibody

Catalog No. ABIN5668203

Product Details anti-F4/80 Antibody

Target: F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))

Antibody Type: Recombinant Antibody

Reactivity: Mouse

Host: Rabbit

Clonality: Chimeric

Conjugate: This F4/80 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC)

Specificity: This antibody recognises the mouse F4/80 antigen, a 160kD glycoprotein, expressed by murine macrophages.

Characteristics:

OriginalSpeciesName: Rat

OriginalFormat: IgG2b

Purification: Purified antibody.

Immunogen: Thioglycollate stimulated peritoneal macrophages of mouse origin.

Clone: Cl:A3-1

Isotype: IgG kappa

Application Details

Application Notes: The anti-F4/80 antibody, clone Cl:A3-1, has been very widely used in flow cytometry, microscopy and Western blotting since its generation by Austyn and Gordon in 1980. We are the only supplier of recombinant versions of Cl:A3-1 which, whilst having the same variable domains as the original, has been shown to block hybridoma derived anti-F4/80:APC binding to in vitro generated bone marrow-derived (BMDMs) and ex vivo splenic murine macrophages. This was tested using both the recombinant version of the original isotype (rat IgG2b) as well as a murinised version (IgG2a, Ab00106-2.0). Further testing on BMDMs shows that our Fc Silent™ rat IgG2b is a valuable new reagent which shows distinct labelling of F4/80-positive cell populations, whilst the isotype control shows no binding, suggesting that only the antigen binding domains are involved in binding, promoting precision of labelling. The murinised versions will be valuable tools in the study of the role of the murine macrophage in vivo without the artefacts rat-derived antigen being co-administered may harbour. Particularly the murine IgG2a (Ab00106-2.0) should be an excellent candidate for macrophage depletion studies, with the Fc Silent™ (Ab00106-2.3) version being the ideal negative control.

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for F4/80

Target: F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))

Alternative Name: F4/80 (EMR1 Products)

Synonyms: EMR1 antibody, TM7LN3 antibody, DD7A5-7 antibody, EGF-TM7 antibody, F4/80 antibody, Gpf480 antibody, Ly71 antibody, adhesion G protein-coupled receptor E1 antibody, ADGRE1 antibody, LOC100424335 antibody, Adgre1 antibody

Background: Mouse macrophage marker, EMR1 receptor, EGF-like module, Cell surface glycoprotein F4/80, EGF-like module receptor 1, Em Gpf4, TM7, Ly-71, f480, f-480, f4 80, f 480, f-4/80, f4-80

UniProt: Q61549