This antibody is expected to recognize all reported isoforms (NP_000556.1, NP_852004.1). The sequence of the immunizing peptide corresponds with a part of the extracellular domain.
Purification
Il6ra Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Il6ra Antibody was raised against synthetic peptide YLLQFQLRYRPVWSK found at an internal region of Il6ra.
IL6RA
Reactivity: Human, Mouse, Rat
FACS, IF (cc), IF (p)
Host: Rabbit
Polyclonal
Alexa Fluor 647
Application Notes
ELISA: antibody detection limit dilution 1:2000. Western Blot: Preliminary experiments in Mouse Spleen and Thymus lysates and in lysates of cell lines NIH3T3 and NSO gave no specific signal but low background (at antibody concentration up to 1μ,g/mL).
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Il6ra is supplied in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Aliquot and store at -20°C. Minimize freezing and thawing.