CRBN antibody (C-Term)

Catalog No. ABIN5689929

Product Details anti-CRBN Antibody

Target: CRBN (Cereblon (CRBN))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CRBN antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: CRBN antibody is human, mouse and rat reactive. At least two isoforms of CRBN are known to exist, this antibody will detect both isoforms.

Purification: CRBN antibody is affinity chromatography purified via peptide column.

Immunogen: CRBN antibody was raised against a 16 amino acid peptide near the carboxy terminus of human CRBN.

The immunogen is located within the last 50 amino acids of CRBN.

Isotype: IgG

Application Details

Application Notes: CRBN antibody can be used for detection of CRBN by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: CRBN antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: CRBN antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for CRBN

Target: CRBN (Cereblon (CRBN))

Alternative Name: CRBN (CRBN Products)

Synonyms: zgc:92404 antibody, mrt2a antibody, F3N11.21 antibody, MRT2 antibody, MRT2A antibody, 2610203G15Rik antibody, 2900045O07Rik antibody, AF229032 antibody, AW108261 antibody, piL antibody, cereblon antibody, ATP-dependent protease La (LON) domain protein antibody, crbn antibody, CRBN antibody, AT2G25740 antibody, Crbn antibody

Background: CRBN, a member of the Lon protease protein family, plays a role in brain development (1). It is a component of the DCX (DDB1-CUL4-X-box) E3 protein ligase complex, a complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins and is required for limb outgrowth and expression of the fibroblast growth factor FGF8 (2). CRBN is thought to regulate the assembly and neuronal surface expression of large-conductance calcium-activated potassium channels in brain regions involved in memory and learning via its interaction with KCNT1. It is widely expressed and highly expressed in brain (3,4).

Molecular Weight: Predicted: 49 kDa

Observed: 54 kDa

Gene ID: 51185

NCBI Accession: NP_057386

UniProt: Q96SW2