PJA1 antibody (AA 50-100)

Catalog No. ABIN5690102

Product Details anti-PJA1 Antibody

Target: PJA1 (Praja Ring Finger 1, E3 Ubiquitin Protein Ligase (PJA1))

Binding Specificity: AA 50-100

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PJA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Specificity: PJA1 antibody is human, mouse and rat reactive. At least two isoforms of PJA1 are known to exist, this antibody will detect both isoforms.

Purification: PJA1 antibody is affinity chromatography purified via peptide column.

Immunogen: PJA1 antibody was raised against a 19 amino acid peptide near the amino terminus of human PJA1.

The immunogen is located within amino acids 50 - 100 of PJA1.

Isotype: IgG

Application Details

Application Notes: PJA1 antibody can be used for detection of PJA1 by Western blot at 0.25 - 0.5 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PJA1 antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: PJA1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for PJA1

Target: PJA1 (Praja Ring Finger 1, E3 Ubiquitin Protein Ligase (PJA1))

Alternative Name: PJA1 (PJA1 Products)

Synonyms: PRAJA1 antibody, RNF70 antibody, praja ring finger ubiquitin ligase 1 antibody, PJA1 antibody, Pja1 antibody

Background: Ubiquitinization is an important cellular degradation process requiring sequential reactions that are mediated by three enzymes: E1, E2 and E3. PJA1, also known as Praja1 and RING finger protein 70, is a 643 amino acid E2-dependent E3-ubiquitin ligase that is abundantly expressed in the brain (1,2). Through interaction and activation with the E2-ubiquitin ligase UBC4, PJA1 mediates substrate-specific ubiquitination via its RING finger domain and facilitates ubiquitination (3). Overexpression of PJA1 in gastrointestinal cancers suggests that it may be responsible for the degradation of some anti-oncogenic proteins (4,5).

Molecular Weight: Predicted: 71 kDa

Observed: 71 kDa

Gene ID: 64219

NCBI Accession: NP_660095

UniProt: Q8NG27