SMARCA4 antibody (AA 1420-1470)

Catalog No. ABIN5690257

Product Details anti-SMARCA4 Antibody

Target: SMARCA4 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, Member 4 (SMARCA4))

Binding Specificity: AA 1420-1470

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMARCA4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Cleavage Under Targets and Release Using Nuclease (CUT&RUN)

Specificity: SMARCA4 antibody is human, mouse and rat reactive. Multiple isoforms of SMARCA4 are known to exist.

Purification: SMARCA4 antibody is affinity chromatography purified via peptide column.

Immunogen: SMARCA4 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human SMARCA4.

The immunogen is located within amino acids 1420 - 1470 of SMARCA4.

Isotype: IgG

Application Details

Application Notes: The rabbit anti-SMARCA4 antibody ABIN6991990 is suitable for use in CUT&RUN, immunofluorescence, immunohistochemistry, immunofluorescence, and Western Blot. Specific conditions for each assay should be optimized by the end user. General dilution recommendations for different applications are as follows:
IHC: 1:500
IF: 1:50
WB: 1:500-1:1,000
CUT&RUN: 1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: SMARCA4 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: SMARCA4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for SMARCA4

Target: SMARCA4 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, Member 4 (SMARCA4))

Alternative Name: SMARCA4 (SMARCA4 Products)

Synonyms: SMARCA4 antibody, BAF190 antibody, BAF190A antibody, BRG1 antibody, MRD16 antibody, RTPS2 antibody, SNF2 antibody, SNF2L4 antibody, SNF2LB antibody, SWI2 antibody, hSNF2b antibody, brg-1 antibody, Brg1 antibody, HP1-BP72 antibody, SNF2beta antibody, SW1/SNF antibody, b2b692Clo antibody, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 antibody, SMARCA4 antibody, Smarca4 antibody

Background: The SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily a member 4 (SMARCA4) protein, also known as BRG1, is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila (1). Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes (2). The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin (3). In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44 (4,5).

Molecular Weight: Predicted: 185 kDa

Observed: 200 kDa

Gene ID: 6597

NCBI Accession: NP_001122321

UniProt: P51532

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Stem Cell Maintenance