SPIB antibody (AA 200-250)

Catalog No. ABIN5690267

Product Details anti-SPIB Antibody

Target: SPIB (Spi-B Transcription Factor (Spi-1/PU.1 Related) (SPIB))

Binding Specificity: AA 200-250

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPIB antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: SPIB antibody is human, mouse and rat reactive. At least four isoforms of SPIB are known to exist, this antibody will detect all but isoform 3.

Purification: SPIB antibody is affinity chromatography purified via peptide column.

Immunogen: SPIB antibody was raised against a 17 amino acid peptide near the carboxy terminus of human SPIB.

The immunogen is located within amino acids 200 - 250 of SPIB.

Isotype: IgG

Application Details

Application Notes: SPIB antibody can be used for detection of SPIB by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: SPIB antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: SPIB antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for SPIB

Target: SPIB (Spi-B Transcription Factor (Spi-1/PU.1 Related) (SPIB))

Alternative Name: SPIB (SPIB Products)

Synonyms: spi-b antibody, spib antibody, spiba antibody, SPI-B antibody, B42b-like antibody, Spi-B antibody, Spi-B transcription factor L homeolog antibody, Spi-B transcription factor antibody, Spi-B transcription factor (Spi-1/PU.1 related) antibody, spib.L antibody, SPIB antibody, Spib antibody

Background: SPIB is a member of the ETS transcription factor family that influences lymphoid development and activity and binds the consensus DNA site GGA (A/T) through a unique winged helix-turn-helix motif known as the ETS domain (1). SPIB is found in hematopoietic cells such as B cells and plasmacytoid dendritic cells (DC) (2,3). It promotes the development of plasmacytoid dendritic cells (pDCs) or natural interferon (IFN)-producing cells. SPIB may be required for B-cell receptor (BCR) signaling, which is necessary for normal B-cell development and antigenic stimulation (3,4).

Molecular Weight: Predicted: 19, 25, 29 kDa

Observed: 20 kDa

Gene ID: 6689

NCBI Accession: NP_003112

UniProt: Q01892