Arylsulfatase A antibody (C-Term) (DyLight 488)

Catalog No. ABIN5692788

This Mouse Monoclonal antibody specifically detects Arylsulfatase A in FACS. It exhibits reactivity toward Human.

Quick Overview for Arylsulfatase A antibody (C-Term) (DyLight 488) (ABIN5692788)

Target: Arylsulfatase A (ARSA)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Arylsulfatase A antibody is conjugated to DyLight 488

Application: Flow Cytometry (FACS)

Clone: 4C10

Product Details anti-Arylsulfatase A Antibody (DyLight 488)

Binding Specificity: AA 454-482, C-Term

Purpose: Anti-Human ARSA DyLight® 488 conjugated Antibody(monoclonal, 4C10)

Sequence: QALKQLQLLK AQLDAAVTFG PSQVARGED

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Human ARSA DyLight® 488 conjugated Antibody (monoclonal, 4C10) (ABIN5692787)-Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Human.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human ARSA, different from the related mouse sequence by six amino acids.

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells1. Fujii T, Kobayashi T, Honke K et al. (1992). "Proteolytic processing of human lysosomal arylsulfatase A". Biochim. Biophys. Acta 1122 (1): 93-8. 2. Kondo R, Wakamatsu N, Yoshino H et al. (1991)."Identification of a mutation in the arylsulfatase A gene of a patient with adult-type metachromatic leukodystrophy". Am. J. Hum. Genet. 48 (5): 971-8. 3. Matzner U, Herbst E, Hedayati KK, Lüllmann-Rauch R, Wessig C, Schröder S, Eistrup C, Möller C, Fogh J, Gieselmann V (May 2005). "Enzyme replacement improves nervous system pathology and function in a mouse model for metachromatic leukodystrophy". Hum. Mol. Genet. 14 (9): 1139-52.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Target Details for Arylsulfatase A

Target: Arylsulfatase A (ARSA)

Alternative Name: ARSA

Background:

Synonyms: Arylsulfatase A, ASA, Cerebroside-sulfatase, Arylsulfatase A component B, Arylsulfatase A component C, ARSA

Background: Arylsulfatase A (ARSA) is an enzyme that breaks down sulfatides, namely cerebroside 3-sulfate intocerebroside and sulfate. In humans, arylsulfatase A is encoded by the ARSA gene. ARSA is mapped to 22q13.33. The protein encoded by this gene hydrolyzes cerebroside sulfate to cerebroside and sulfate. Defects in this gene lead to metachromatic leucodystrophy (MLD), a progressive demyelination disease which results in a variety of neurological symptoms and ultimately death. Alternatively spliced transcript variants have been described for this gene.

Molecular Weight: 39 kDa

Gene ID: 410

UniProt: P15289