PDGFRB antibody (AA 911-992)

Catalog No. ABIN5693171

Product Details anti-PDGFRB Antibody

Target: PDGFRB (Platelet Derived Growth Factor Receptor beta (PDGFRB))

Binding Specificity: AA 911-992

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDGFRB antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: Picoband™

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for PDGFRB detection. Tested with WB, IHC-P, Direct ELISA in Human,Mouse,Rat.

Immunogen: E. coli-derived human PDGFRB recombinant protein (Position: E911-L992).

Application Details

Application Notes:

Recommended Detection Systems: Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Application Details: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry(Paraffin-embedded Section), 0.5-1 μg/mL
Direct ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

References for anti-PDGFRB antibody (ABIN5693171)

Li, Dai, Zhao, Zhang, Hui, Zhang, Jin: "Preventative effect of Astragalus flavescens on hepatic fibrosis in rats and its mechanism of action." in: Experimental and therapeutic medicine, Vol. 6, Issue 4, pp. 904-908, (2013) (PubMed).

Chen, Zhang, Wang, Chen, Xie, Chen: "RNA interference targeting the platelet-derived growth factor receptor beta subunit ameliorates experimental hepatic fibrosis in rats." in: Liver international : official journal of the International Association for the Study of the Liver, Vol. 28, Issue 10, pp. 1446-57, (2009) (PubMed).

Target Details for PDGFRB

Target: PDGFRB (Platelet Derived Growth Factor Receptor beta (PDGFRB))

Alternative Name: PDGFRB (PDGFRB Products)

Synonyms: AI528809 antibody, CD140b antibody, PDGFR-1 antibody, Pdgfr antibody, CD140B antibody, IBGC4 antibody, JTK12 antibody, PDGFR antibody, PDGFR1 antibody, platelet derived growth factor receptor, beta polypeptide antibody, platelet derived growth factor receptor beta antibody, Pdgfrb antibody, PDGFRB antibody

Background:

Synonyms: Platelet-derived growth factor receptor beta, PDGF-R-beta, PDGFR-beta, Beta platelet-derived growth factor receptor, Beta-type platelet-derived growth factor receptor, CD140 antigen-like family member B, Platelet-derived growth factor receptor 1, PDGFR-1, CD140b, PDGFRB, PDGFR, PDGFR1

Background: Beta-type platelet-derived growth factor receptor is a protein that in humans is encoded by the PDGFRB gene. The protein encoded by this gene is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer (PDGFB or PDGFD) or a heterodimer (PDGFA and PDGFB). This gene is essential for normal development of the cardiovascular system and aids in rearrangement of the actin cytoskeleton. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor, all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the ETV6 gene, results in chronic myeloproliferative disorder with eosinophilia.

UniProt: P09619

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling