ITGA5 antibody (AA 42-263)

Catalog No. ABIN5693202

Product Details anti-ITGA5 Antibody

Target: ITGA5 (Integrin, alpha 5 (ITGA5))

Binding Specificity: AA 42-263

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ITGA5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Brand: Picoband™

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Integrin alpha 5 detection. Tested with WB, IHC-P, Direct ELISA in Human,Mouse,Rat.

Immunogen: E. coli-derived human Integrin alpha 5 recombinant protein (Position: F42-A263).

Application Details

Application Notes:

Recommended Detection Systems: Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Application Details: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry(Paraffin-embedded Section), 0.5-1 μg/mL
Direct ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

References for anti-ITGA5 antibody (ABIN5693202)

Chen, Sun, Liu, Tong, Meng: "Silencing of Paternally Expressed Gene 10 Inhibits Trophoblast Proliferation and Invasion." in: PLoS ONE, Vol. 10, Issue 12, pp. e0144845, (2015) (PubMed).

Zhang, Ren, Luo, Fan, Liu, Liang, Ma, Yu, Bai: "A small physiological electric field mediated responses of extravillous trophoblasts derived from HTR8/SVneo cells: involvement of activation of focal adhesion kinase signaling." in: PLoS ONE, Vol. 9, Issue 3, pp. e92252, (2014) (PubMed).

Huang, Lian, Chen, Yang, Yang: "Characterization of rat hair follicle stem cells selected by vario magnetic activated cell sorting system." in: Acta histochemica et cytochemica, Vol. 42, Issue 5, pp. 129-36, (2009) (PubMed).

"College training and examinations." in: The Australian and New Zealand journal of psychiatry, Vol. 23, Issue 2, pp. 148-54, (1989) (PubMed).

Target Details for ITGA5

Target: ITGA5 (Integrin, alpha 5 (ITGA5))

Alternative Name: ITGA5 (ITGA5 Products)

Synonyms: ITGA5 antibody, Cd49e antibody, Fnra antibody, VLA5 antibody, CD49e antibody, FNRA antibody, VLA5A antibody, Itga5_retired antibody, integrin subunit alpha 5 antibody, integrin alpha 5 (fibronectin receptor alpha) antibody, ITGA5 antibody, Itga5 antibody

Background:

Synonyms: Integrin alpha-5, CD49 antigen-like family member E, Fibronectin receptor subunit alpha, Integrin alpha-F, VLA-5, CD49e, Integrin alpha-5 heavy chain, Integrin alpha-5 light chain, ITGA5, FNRA

Background: Integrin alpha-5, also known as FNRA or VLA5A, is a protein that in humans is encoded by the ITGA5 gene. The product of this gene belongs to the integrin alpha chain family. Integrins are integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.

UniProt: P08648

Pathways: Integrin Complex