Spt6 antibody

Catalog No. ABIN5703155

Product Details anti-Spt6 Antibody

Target: Spt6 (SUPT6H) (Suppressor of Ty 6 Homolog (SUPT6H))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Spt6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Immunogen: suppressor of Ty 6 homolog (S. cerevisiae)

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol  pH 7.3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Expiry Date: 12 months

Target Details for Spt6

Target: Spt6 (SUPT6H) (Suppressor of Ty 6 Homolog (SUPT6H))

Alternative Name: SUPT6H (SUPT6H Products)

Synonyms: SPT6 antibody, SPT6H antibody, emb-5 antibody, 5131400N11Rik antibody, AI132449 antibody, Supt6h antibody, mKIAA0162 antibody, Spt6 antibody, wu:fj42h11 antibody, SUPT6H antibody, spt6 antibody, spt6h antibody, MGC146884 antibody, SPT6 homolog, histone chaperone antibody, suppressor of Ty 6 antibody, SUPT6H,-like protein to suppressor of ty 6 antibody, transcription elongation factor SPT6 antibody, suppressor of Ty 6 homolog (S. cerevisiae) antibody, chromatin-remodeling histone chaperone antibody, SUPT6H antibody, Supt6 antibody, Supt6h antibody, supt6h antibody, LOC100092175 antibody, SPT6 antibody

Background: Synonyms:KIAA0162, SPT6H Background:Transcription elongation factor which binds histone H3 and plays a key role in the regulation of transcription elongation and mRNA processing. Enhances the transcription elongation by RNA polymerase II(RNAPII) and is also required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. Besides chaperoning histones in transcription, acts to transport and splice mRNA by forming a complex with IWS1 and the C-terminal domain(CTD) of the RNAPII subunit RPB1(POLR2A). The SUPT6H:IWS1:CTD complex recruits mRNA export factors(ALYREF/THOC4, EXOSC10) as well as histone modifying enzymes(such as SETD2), to ensure proper mRNA splicing, efficient mRNA export and elongation-coupled H3K36 methylation, a signature chromatin mark of active transcription. SUPT6H via its association with SETD1A, regulates both class-switch recombination and somatic hypermutation through formation of H3K4me3 epigenetic marks on activation-induced cytidine deaminase(AICDA) target loci. Promotes the activation of the myogenic gene program by entailing erasure of the repressive H3K27me3 epigenetic mark through stabilization of the chromatin interaction of the H3K27 demethylase KDM6A.

Gene ID: 6830

UniProt: Q7KZ85

Pathways: Regulation of Leukocyte Mediated Immunity, Regulation of Muscle Cell Differentiation, Production of Molecular Mediator of Immune Response