Histone 3 antibody (H3K27me3, pSer28)
(1 reference)-
- Target See all Histone 3 (H3) Antibodies
- Histone 3 (H3)
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Binding Specificity
- H3K27me3, pSer28
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Reactivity
- Human, Mouse, Rat, Chicken, Xenopus laevis, C. elegans, Drosophila melanogaster, Plant
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Histone 3 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Chromatin Immunoprecipitation (ChIP), Fluorescence Microscopy (FM)
- Characteristics
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Synonyms: rabbit anti-Histone H3 trimethyl Lys18 pS28 antibody, H3.3B, H3pS28K18Me3, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87783, MGC87782, H3 K27me3/pS28
Background: Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Correspondingly, chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is a very dynamic structure in which numerous post-translational modifications work together to activate or repress the availability of DNA to be copied, transcribed, or repaired. These marks decide which DNA will be open and commonly active (euchromatin) or tightly wound to prevent access and activation (heterochromatin). Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. Specifically, trimethylation of K27 is associated with gene silencing, whereas pS28 is associated with mitotis and immediate early genes. Anti-Histone H3 are ideal for researchers interested in Chromatin Modifiers, Chromatin Research, Histones and Modified Histones, and Epigenetics research.
Gene Name: HIST2H3C
- Purification
- Anti-Histone H3 [Trimethyl Lys27, p Ser28] was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H3. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.
- Immunogen
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Immunogen: Histone H3 [Trimethyl Lys27, p Ser28] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with synthetic trimethylated/phosphorylated peptides surrounding Lysine 27 and Serine 28 of human Histone H3.
Immunogen Type: Peptide
- Isotype
- IgG
- Top Product
- Discover our top product H3 Primary Antibody
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anti-Histone 3 (H3) (H3K4me) antibody
H3 Reactivity: Human WB, IF, ChIP, IP, ChIP-seq, CUT&RUN Host: Rabbit Polyclonal unconjugated
anti-Histone 3 (H3) (H3K27ac) antibodyH3 Reactivity: Human, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated
anti-Histone 3 (H3) (3meLys4) antibodyH3 Reactivity: Human, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN Host: Rabbit Polyclonal unconjugated
anti-Histone 3 (H3) (H3K36me3) antibodyH3 Reactivity: Human, Mouse WB, ChIP, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Mouse Monoclonal MABI 0333 unconjugated
anti-Histone 3 (H3) (H3K9ac) antibodyH3 Reactivity: Human, Mouse WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated
anti-Histone 3 (H3) (AA 71-136) antibodyH3 Reactivity: Human, Mouse, Rat WB, ELISA, ICC, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-Histone 3 (H3) (H3K27me) antibodyH3 Reactivity: Human WB, IHC, IF, ChIP, IP, ChIP-seq Host: Rabbit Polyclonal unconjugated
anti-Histone 3 (H3) (H3K4me3) antibodyH3 Reactivity: Human, Mouse, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated
anti-Histone 3 (H3) (C-Term) antibodyH3 Reactivity: Human WB, IHC, ChIP, IP Host: Rabbit Polyclonal unconjugated
anti-Histone 3 (H3) (H3K4me3) antibodyH3 Reactivity: Human WB, IHC, IF, ChIP, IP, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated
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- Application Notes
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Immunohistochemistry Dilution: 1:2000
Application Note: Anti-Histone H3 [Trimethyl Lys27, p Ser28] antibody is useful for Western Blot, Chromatin Immunoprecipitation, Dot Blot, and Immunocytochemistry/Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.
ChIP Dilution: 2-5 μg/million cells
Western Blot Dilution: 1 μg/mL
IF Microscopy Dilution: 1:2000
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.66 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 30 % Glycerol
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Expiry Date
- 12 months
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: "Epigenetic Changes at the Birc5 Promoter Induced by YM155 in Synovial Sarcoma." in: Journal of clinical medicine, Vol. 8, Issue 3, (2019) (PubMed).
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: "Epigenetic Changes at the Birc5 Promoter Induced by YM155 in Synovial Sarcoma." in: Journal of clinical medicine, Vol. 8, Issue 3, (2019) (PubMed).
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- Target
- Histone 3 (H3)
- Alternative Name
- Histone H3 (H3 Products)
- Synonyms
- H-3 antibody, histocompatibility 3 antibody, H3 antibody
- Gene ID
- 126961
- NCBI Accession
- NP_001005464
- UniProt
- Q71DI3
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