Histone H4 antibody (meLys20)
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- Target See all Histone H4 Antibodies
- Histone H4
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Binding Specificity
- meLys20
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Reactivity
- Human, Mouse, Rat, Chicken, Xenopus laevis, Drosophila melanogaster, C. elegans, Plant
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Histone H4 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Chromatin Immunoprecipitation (ChIP), Fluorescence Microscopy (FM)
- Characteristics
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Synonyms: rabbit anti-Histone H4 monomethyl Lys20 antibody, H4K20me1, HIST2H4B, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4, HIST2H4A, HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, histone cluster 4, H4, histone 4, H4
Background: SET8 specifically catalyzes the mono-methylation of H4 at K20. Loss of this crucial methylation causes multiple DNA breaks, which instigates a p53-dependent DNA damage response to avoid mitosis and aberrant chromosomal activity. Therefore, this PTM is essential to genome replication and stability through S-phase. In mammalian stem cells, Xist expression blocks the formation of H4K20me1, which is one of the first examples of a direct connection between chromatin and stem cell differentiation. Anti-Histone H4 are ideal for researchers interested in Chromatin Modifiers, Chromatin Research, DNA Repair, DNA replication Transcription Translation and Splicing, Histones and Modified Histones, and Epigenetics research.
Gene Name: HIST4H4
- Purification
- Anti-Histone H4 [Monomethyl Lys20] was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H4. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with most mammal species. Cross-reactivity with Histone H4 from other sources has not been determined.
- Immunogen
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Immunogen: Histone H4 [Monomethyl Lys20] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic monomethylated peptide surrounding Lysine 20 of human Histone H4.
Immunogen Type: Peptide
- Isotype
- IgG
- Top Product
- Discover our top product Histone H4 Primary Antibody
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- Application Notes
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Immunohistochemistry Dilution: 1:50
Application Note: Anti-Histone H4 [Monomethyl Lys20] antibody is useful for Western Blot, Dot Blot, Chromatin Immunoprecipitation, Immunofluorescence, and Immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~13 kDa corresponding to Histone H4 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.
ChIP Dilution: 2-5 μg/million cells
Western Blot Dilution: 1:500
IF Microscopy Dilution: 1:50
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.12 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 30 % Glycerol
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Expiry Date
- 12 months
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ATR kinase inhibition induces unscheduled origin firing through a Cdc7-dependent association between GINS and And-1." in: Nature communications, Vol. 8, Issue 1, pp. 1392, (2018) (PubMed).
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ATR kinase inhibition induces unscheduled origin firing through a Cdc7-dependent association between GINS and And-1." in: Nature communications, Vol. 8, Issue 1, pp. 1392, (2018) (PubMed).
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- Target
- Histone H4
- Abstract
- Histone H4 Products
- Synonyms
- hist1h4l antibody, H1ft antibody, Hist4 antibody, Hist4h4 antibody, H4 antibody, X04652 antibody, FO108 antibody, H4/n antibody, H4F2 antibody, H4FN antibody, HIST2H4 antibody, H4C13 antibody, H4C7 antibody, F8N16.2 antibody, F8N16_2 antibody, histone H4 antibody, BcDNA:RH52884 antibody, CG3379 antibody, Dmel\CG3379 antibody, H4r antibody, His4-88CD antibody, His4R antibody, histone cluster 1, H4a L homeolog antibody, histone cluster 1 H4 family member B antibody, histone cluster 2, H4 antibody, histone cluster 2 H4 family member a antibody, histone H4 antibody, Histone H4 replacement antibody, Histone H4 antibody, hist1h4a.L antibody, Hist1h4b antibody, Hist2h4 antibody, HIST2H4A antibody, H4 antibody, H4C14 antibody, HIS4 antibody, HIST2H4 antibody, His4r antibody, his-38 antibody
- Gene ID
- 121504
- NCBI Accession
- NP_001029249
- UniProt
- P62805
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