NFkB antibody (acLys310)

Catalog No. ABIN5706804

Product Details anti-NFkB Antibody

Target: NFkB (Nuclear Factor kappa B (NFkB))

Binding Specificity: acLys310

Reactivity: Human, Mouse, Rat, Chicken, Primate

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFkB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Characteristics:

Synonyms: rabbit anti-NFKB Ac Lys310 antibody, rabbit anti-NfKB K310ac antibody, NFKB, NFkß, NF-kB, NF-kappaB, NFkappaB, Transcription factor p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, RELA, NFKB3

Background: NFkB is a pleiotropic transcription factor present in most cell types and is the endpoint of a series of signal transduction events related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorgenesis and apoptosis. NFkB is contained in the cytoplasm inactively until it is released by a phosphorylated I-kappa-B, allowing the NFkB to translocate to the nucleus. This antibody is ideal for investigators interested in Metabolism, Cancer, Cardiovascular, Microbiology, Epigenetics and Nuclear Signaling, Immunology, Neuroscience, or Cell Biology research.

Gene Name: RELA

Purification: Anti-NFkB [ac Lys 310] Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with mouse and human based on 100% sequence homology. Cross-reactivity with NFkB K310 ac from other sources has not been determined.

Immunogen:

Immunogen: Anti-NFkB K310ac affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic acetylated peptide surrounding Lysine 310 of human TF65 protein.

Immunogen Type: Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-NFkB [ac Lys 310] Antibody has been tested in ELISA. Anti-NFkB K310ac is useful for in Immunohistochemistry and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~65  kDa corresponding to the appropriate cell lysate or extract.

Western Blot Dilution: 1 μg/mL

ELISA Dilution: 1: 50,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.05 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,  pH 7.2

Stabilizer: 50 % (v/v) Glycerol with 1 mg/mL Bovine Serum Albumin (BSA)

Preservative: Without preservative

Storage: 4 °C,-20 °C

Expiry Date: 12 months

Target Details for NFkB

Target: NFkB (Nuclear Factor kappa B (NFkB))

Alternative Name: NFkB (NFkB Products)

Synonyms: EBP-1 antibody, KBF1 antibody, NF-kB1 antibody, NF-kappa-B antibody, NF-kappaB antibody, NFKB-p105 antibody, NFKB-p50 antibody, NFkappaB antibody, p105 antibody, p50 antibody, NF-kB antibody, NF-KB1 antibody, NF-kappaB1 antibody, p50/p105 antibody, nuclear factor kappa B subunit 1 antibody, nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 antibody, NFKB1 antibody, Nfkb1 antibody

Pathways: Ubiquitin Proteasome Pathway, S100 Proteins