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Spartin (SPART) (Internal Region) antibody

Verified SPART Reactivity: Human ELISA, IF, WB Host: Goat Polyclonal unconjugated
Catalog No. ABIN570717
  • Target
    Spartin (SPART)
    Binding Specificity
    Internal Region
    Reactivity
    Human
    Host
    • 1
    Goat
    Clonality
    • 1
    Polyclonal
    Conjugate
    • 1
    Un-conjugated
    Application
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunofluorescence (IF), Western Blotting (WB)
    Purpose
    SPARTIN
    Sequence
    EASGTDVKQL DQGNK
    Cross-Reactivity
    Human
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence C-EASGTDVKQLDQGNK, from the internal region of the protein sequence according to NP_055902.1.
    Isotype
    IgG
  • Application Notes

    Western Blot: Approx 70 kDa band observed in lysates of cell line HeLa (calculated MW of 72.8 kDa according to NP_055902.1). Recommended concentration: 1-3 μg/mL.

    Peptide ELISA: antibody detection limit dilution 1:8000.

    Comment

    Immunofluorescence: HeLa show dimished signals after knock down of expresssion by introduction of siRNA.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    Spartin (SPART)
    Alternative Name
    SPART
    Background
    SPG20, spastic paraplegia 20 (Troyer syndrome), KIAA0610, SPARTIN, TAHCCP1, OTTHUMP00000018254, spastic paraplegia 20, spastic paraplegia 20, spartin (Troyer syndrome), trans-activated by hepatitis C virus core protein 1
    Gene ID
    23111
    NCBI Accession
    NP_055902
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