CDC45 antibody

Catalog No. ABIN5708443

Product Details anti-CDC45 Antibody

Target: CDC45 (Cell Division Cycle 45 Homolog (S. Cerevisiae) (CDC45))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC45 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Cross-Reactivity (Details): Expected species reactivity: Human

Purification: Antigen affinity purified

Immunogen: A recombinant human protein corresponding to amino acids E166-A431 was used as the immunogen for the CDC45 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the CDC45 antibody should be determined by the researcher.\. Western blot: 0.5-1 μg/mL, Direct ELISA: 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the CDC45 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for CDC45

Target: CDC45 (Cell Division Cycle 45 Homolog (S. Cerevisiae) (CDC45))

Alternative Name: CDC45 / PORC-PI-1 (CDC45 Products)

Synonyms: cdc45l antibody, cdc45l2 antibody, porc-pi-1 antibody, CDC45L antibody, CDC45L2 antibody, PORC-PI-1 antibody, Cdc45l antibody, cell division cycle 45 antibody, cell division cycle 45 S homeolog antibody, cell division cycle 45 L homeolog antibody, cdc45 antibody, CDC45 antibody, cdc45.S antibody, Cdc45 antibody, cdc45.L antibody

Background: Cell division control protein 45 homolog was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Alternate splicing results in multiple transcript variants.

UniProt: O75419

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA