PPP2R4 antibody (C-Term)

Catalog No. ABIN570992

Product Details anti-PPP2R4 Antibody

Target: PPP2R4 (Protein Phosphatase 2A Activator, Regulatory Subunit 4 (PPP2R4))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This PPP2R4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Purpose: PPP2R4 / PP2A

Sequence: GSLLPIHPVT SG

Specificity: This antibody is expected to recognize all reported isoforms (NP_821068.1, NP_066954.2, NP_821070.1). Reported variants NP_066954.2 and NP_821067.1 represent identical protein.

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-GSLLPIHPVTSG, from the C Terminus of the protein sequence according to NP_821068.1, NP_066954.2, NP_821070.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Testis. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 37 kDa band observed in Human, Mouse and Rat Liver lysates and in lysates of cell line HepG2 (calculated MW of 36.8 kDa according to NP_066954.2). Recommended concentration: 0.5-2 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PPP2R4

Target: PPP2R4 (Protein Phosphatase 2A Activator, Regulatory Subunit 4 (PPP2R4))

Alternative Name: PTPA (PPP2R4 Products)

Synonyms: PP2A antibody, PR53 antibody, PTPA antibody, 2610042B21Rik antibody, C77440 antibody, N28142 antibody, PTPRA antibody, Ppp2r4 antibody, fc23d07 antibody, ppp2r4 antibody, wu:fc23d07 antibody, zgc:92766 antibody, pp2a antibody, ppp2r4.L antibody, pr53 antibody, ptpa antibody, protein phosphatase 2 phosphatase activator antibody, protein phosphatase 2 protein activator antibody, protein phosphatase 2 phosphatase activator L homeolog antibody, PTPA antibody, Ptpa antibody, ptpa antibody, ptpa.L antibody

Background: PPP2R4, protein phosphatase 2A activator, regulatory subunit 4, MGC2184, PP2A, PR53, PTPA, OTTHUMP00000022333, PP2A phosphatase activator, PP2A, subunit B', phosphotyrosyl phosphatase activator, protein phosphatase 2A, regulatory subunit B', protein phosp

Gene ID: 5524, 110854, 362102

NCBI Accession: NP_821068, NP_066954, NP_821070

Pathways: PI3K-Akt Signaling, M Phase, Hepatitis C, Toll-Like Receptors Cascades