TRIM29 antibody (Internal Region)

Catalog No. ABIN571104

Product Details anti-TRIM29 Antibody

Target: TRIM29 (Tripartite Motif Containing 29 (TRIM29))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This TRIM29 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose: TRIM29 / ATDC

Sequence: EKDRIKSFTT NE

Cross-Reactivity: Cow, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-EKDRIKSFTTNE, from the internal region of the protein sequence according to NP_036233.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Skin shows staining of subnuclear structures and cytoplasm in keratinocytes. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 37 kDa band observed in Human Peripheral Blood Mononucleocytes (PBM) and in lysates of cell lines HeLa. An additional 70 kDa band was seen in some PBM lysates (calculated MW of 37.3 kDa according to NP_001317311.1 (isoform 2) and 65.8

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the Cytoplasm and Intermediate filaments of A431 cells. Strong expression of the protein seen in the Nucleus of U2OS cells. Recommended concentration: 10μg/ml.

Flow C

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for TRIM29

Target: TRIM29 (Tripartite Motif Containing 29 (TRIM29))

Alternative Name: TRIM29 (TRIM29 Products)

Synonyms: ATDC antibody, 1110047J21Rik antibody, 2810431N19Rik antibody, 4732461M22Rik antibody, AI119726 antibody, MGC53484 antibody, TRIM29 antibody, atdc antibody, tripartite motif containing 29 antibody, tripartite motif-containing 29 antibody, tripartite motif containing 29 L homeolog antibody, TRIM29 antibody, Trim29 antibody, trim29.L antibody, trim29 antibody

Background: TRIM29, tripartite motif-containing 29, ATDC, FLJ36085, ataxia-telangiectasia group D-associated protein, tripartite motif protein TRIM29

Molecular Weight: 65.8kDa

Gene ID: 23650, 72169, 300656

NCBI Accession: NP_036233, NP_001317311

Pathways: p53 Signaling, Apoptosis, DNA Damage Repair, Inositol Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus