TRIM71 antibody (AA 409-419)

Catalog No. ABIN571138

Product Details anti-TRIM71 Antibody

Target: TRIM71 (Tripartite Motif Containing 71, E3 Ubiquitin Protein Ligase (TRIM71))

Binding Specificity: AA 409-419

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This TRIM71 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Purpose: TRIM71 (aa409-419)

Sequence: NKLESTISAV Q

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-NKLESTISAVQ , from the internal region of the protein sequence according to NP_001034200.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 100 kDa band observed in lysates of cell line NIH3T3 (calculated MW of 93.4 kDa according to Human NP_001034200.1 and of 92.1 kDa according to Mouse NP_001035968.1). Recommended concentration: 1-3 μg/mL. An additional band of unknown ide

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for TRIM71

Target: TRIM71 (Tripartite Motif Containing 71, E3 Ubiquitin Protein Ligase (TRIM71))

Alternative Name: TRIM71 (TRIM71 Products)

Background: TRIM71, tripartite motif-containing 71, LIN-41, LIN41, abnormal cell LINeage LIN-41, homolog of C. elegans Lin-41

Gene ID: 131405, 636331, 301042

NCBI Accession: NP_001034200

Pathways: Tube Formation