CD34 antibody

Catalog No. ABIN571353

Product Details anti-CD34 Antibody

Target: CD34

Reactivity: Human, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD34 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: The mouse monoclonal antibody 581 reacts with an extracellular epitope of CD34, a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells, it is gradually lost on progenitor cells. The antibody recognizes the class III CD34 epitope resistant to neuraminidase, chymopapain and glycoprotease.

No Cross-Reactivity: Sheep

Cross-Reactivity (Details): Human, Non-Human Primates

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Clone: 581

Isotype: IgG1 kappa

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-4 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-CD34 antibody (ABIN571353)

Goardon, Nikolousis, Sternberg, Chu, Craddock, Richardson, Benson, Drayson, Standen, Vyas, Freeman: "Reduced CD38 expression on CD34+ cells as a diagnostic test in myelodysplastic syndromes." in: Haematologica, Vol. 94, Issue 8, pp. 1160-3, (2009) (PubMed).

Ninos, Jefferies, Cogle, Kerr: "The thrombopoietin receptor, c-Mpl, is a selective surface marker for human hematopoietic stem cells." in: Journal of translational medicine, Vol. 4, pp. 9, (2006) (PubMed).

Ono, Sharma, Smith, Burnett, Aurelian: "CD34+ cells in the peripheral blood transport herpes simplex virus DNA fragments to the skin of patients with erythema multiforme (HAEM)." in: The Journal of investigative dermatology, Vol. 124, Issue 6, pp. 1215-24, (2005) (PubMed).

Kato, Ando, Nakamura, Muguruma, Sato, Yabe, Yabe, Hattori, Yasuda, Hotta: "Absence of a CD34- hematopoietic precursor population in recipients of CD34+ stem cell transplantation." in: Bone marrow transplantation, Vol. 28, Issue 6, pp. 587-95, (2001) (PubMed).

Felschow, McVeigh, Hoehn, Civin, Fackler: "The adapter protein CrkL associates with CD34." in: Blood, Vol. 97, Issue 12, pp. 3768-75, (2001) (PubMed).

Ando, Nakamura, Chargui, Matsuzawa, Tsuji, Kato, Hotta: "Extensive generation of human cord blood CD34(+) stem cells from Lin(-)CD34(-) cells in a long-term in vitro system." in: Experimental hematology, Vol. 28, Issue 6, pp. 690-9, (2000) (PubMed).

Fluckiger, Sanz, Garcia-Lloret, Su, Hao, Kato, Quan, de la Hera, Crooks, Witte, Rawlings: "In vitro reconstitution of human B-cell ontogeny: from CD34(+) multipotent progenitors to Ig-secreting cells." in: Blood, Vol. 92, Issue 12, pp. 4509-20, (1999) (PubMed).

Janowska-Wieczorek, Marquez, Nabholtz, Cabuhat, Montaño, Chang, Rozmus, Russell, Edwards, Turner: "Growth factors and cytokines upregulate gelatinase expression in bone marrow CD34(+) cells and their transmigration through reconstituted basement membrane." in: Blood, Vol. 93, Issue 10, pp. 3379-90, (1999) (PubMed).

Target Details for CD34

Target: CD34

Alternative Name: CD34 (CD34 Products)

Synonyms: AU040960 antibody, CD34 molecule antibody, CD34 antigen antibody, CD34 antibody, Cd34 antibody

Background: CD34 Molecule,CD34 is a highly glycosylated monomeric 111-115 kDa surface protein, which is present on many stem cell populations. It is a well established stem cell marker, though its expression on human hematopoietic stem cells is reversible. CD34 probably serves as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions.

Gene ID: 947

UniProt: P28906