NG2 antibody (Extracellular Domain)

Catalog No. ABIN571935

Product Details anti-NG2 Antibody

Target: NG2 (MCSP) (Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP))

Binding Specificity: Extracellular Domain

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NG2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: Specific for NG2.

Cross-Reactivity: Mouse

Cross-Reactivity (Details): Other species not yet tested.

Purification: IgG

Immunogen: A synthetic peptide from extracellular domain of mouse NG2 conjugated to blue carrier protein was used as the antigen.

Isotype: IgG

Application Details

Application Notes: IHC, WB. A concentration of 10-50 μg,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 μL of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for NG2

Target: NG2 (MCSP) (Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP))

Alternative Name: NG2 (MCSP Products)

Synonyms: HMW-MAA antibody, MCSP antibody, MCSPG antibody, MEL-CSPG antibody, MSK16 antibody, NG2 antibody, 4732461B14Rik antibody, AN2 antibody, Ng2 antibody, HSMCSGEN1 antibody, MCS antibody, chondroitin sulfate proteoglycan 4 antibody, sperm mitochondria associated cysteine rich protein antibody, CSPG4 antibody, Cspg4 antibody, SMCP antibody

Background: Valuable marker for several incompletely differentiated precursor cells. Function: Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades. Subcellular location: Apical cell membrane, Single-pass type I membrane protein, Extracellular side. Cell projection âÄ° lamellipodium membrane, Single-pass type I membrane protein, Extracellular side. Tissue specificity: Expressed in microcascular pericytes and not endothelial cells.,Neurogenesis,MEL CSPG, MELCSPG, Chondroitin sulfate proteoglycan 4, Chondroitin sulfate proteoglycan NG2, CSPG4, MCSP, MCSPG, Melanoma associated chondroitin sulfate proteoglycan, Melanoma chondroitin sulfate proteoglycan, MSK16

UniProt: Q8VHY0

Pathways: Glycosaminoglycan Metabolic Process