ROBO1 antibody (Roundabout, Axon Guidance Receptor, Homolog 1 (Drosophila)) (C-Term)

Catalog No. ABIN572076

Product Details anti-ROBO1 Antibody

Target: ROBO1

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Binding Specificity: C-Term

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• Internal Region 9
• AA 1632-1644 4
• AA 29-143 4
• AA 491-589 3
• AA 1097-1130 2
• AA 1275-1325 1
• AA 19-560 1
• AA 264-897 1
• AA 491-590 1
• AA 730-810 1
• C-Term 1
• Center 1

Reactivity: Mouse

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• Human 35
• Mouse 14
• Rat 14
• Dog 3
• Rabbit 1

Host: Rabbit

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• Rabbit 23
• Goat 8
• Mouse 6

Clonality: Polyclonal

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• Polyclonal 32
• Monoclonal 5

Conjugate: This ROBO1 antibody is un-conjugated

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• Un-conjugated 21
• Biotin 3
• FITC 2
• HRP 2
• APC 1
• Alexa Fluor 488 1
• Alexa Fluor 555 1
• Alexa Fluor 594 1
• Alexa Fluor 647 1
• Alexa Fluor 680 1
• Alexa Fluor 750 1
• Alkaline Phosphatase (AP) 1
• PE 1

Application: Immunohistochemistry (IHC), Western Blotting (WB)

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• Western Blotting (WB) 28
• ELISA 24
• Immunohistochemistry (IHC) 5
• Flow Cytometry (FACS) 2
• Immunofluorescence (IF) 2
• Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 2
• Immunoprecipitation (IP) 2
• Enzyme Immunoassay (EIA) 1
• Immunofluorescence (fixed cells) (IF/ICC) 1

Specificity: Specific for Robo1.

Purification: Whole serum

Immunogen: A synthetic peptide to from c-terminal region of mouse Robo1 (Roundabout homolog 1) conjugated to an immunogenic carrier protein was used as the antigen.

Application Details

Application Notes: A dilution of 1: 300 to 1: 2000 is recommended.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for ROBO1

Target: ROBO1

Alternative Name: Robo1 (ROBO1 Antibody Abstract)

Synonyms: DUTT1, SAX3, AW494633, AW742721, Gm310, ROBO1, dutt1, roundabout, sax3, MGC122264, roundabout-1, roundabout guidance receptor 1, roundabout, axon guidance receptor, homolog 1 (Drosophila), roundabout guidance receptor 1 L homeolog, ROBO1, Robo1, robo1, robo1.L

Background: Function: Receptor for SLIT1 and SLIT2 which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. May be required for lung development.
Subcellular location: Membrane, Single-pass type I membrane protein
Tissue specificity: Expressed in embryonal spinal chord. Expressed in embryonal lung, and in adult lung bronchial epithelial cells of large proximal airways. Also known as: Roundabout homolog 1, Dutt1.

Pathways: Positive Regulation of Endopeptidase Activity