SFRP1 antibody (AA 302-314)

Catalog No. ABIN574056

Product Details anti-SFRP1 Antibody

Target: SFRP1 (Secreted Frizzled-Related Protein 1 (SFRP1))

Binding Specificity: AA 302-314

Reactivity: Human, Mouse, Rat, Cow, Horse, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This SFRP1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human SFRP1.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Horse, Opossum (100%) Hamster (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide KNHECPTFQSVFK from the C-terminus of human SFRP1 (NP_003003.3). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Horse, Opossum (100%), Hamster (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for SFRP1

Target: SFRP1 (Secreted Frizzled-Related Protein 1 (SFRP1))

Alternative Name: SFRP1 (SFRP1 Products)

Synonyms: FRP antibody, FRP-1 antibody, FRP1 antibody, FrzA antibody, SARP2 antibody, 2210415K03Rik antibody, AW011917 antibody, AW107218 antibody, AW742929 antibody, sFRP-1 antibody, CSFRP1 antibody, SFRP1 antibody, XfrzA antibody, frp antibody, frp-1 antibody, frp1 antibody, frzA antibody, sarp2 antibody, sfrp1 antibody, LOC100231868 antibody, Srfp1a antibody, wu:fd19b09 antibody, secreted frizzled related protein 1 antibody, secreted frizzled-related protein 1 antibody, Secreted frizzled-related protein 1 antibody, secreted frizzled-related protein 1a antibody, secreted frizzled-related protein 1b antibody, SFRP1 antibody, Sfrp1 antibody, sfrp1 antibody, sfrp1a antibody, sfrp1b antibody

Background: Name/Gene ID: SFRP1
Subfamily: Frizzled

Synonyms: SFRP1, FrzA, FRP, SARP-2, SARP2, SFRP-1, FRP-1, FRP1

Gene ID: 6422

NCBI Accession: NP_003003

UniProt: Q8N474

Pathways: WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Negative Regulation of Hormone Secretion, Regulation of Intracellular Steroid Hormone Receptor Signaling, Stem Cell Maintenance, Tube Formation, Positive Regulation of fat Cell Differentiation