PARP3 antibody (AA 90-104)

Catalog No. ABIN574304

Product Details anti-PARP3 Antibody

Target: PARP3 (Poly (ADP-Ribose) Polymerase Family, Member 3 (PARP3))

Binding Specificity: AA 90-104

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PARP3 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human PARP3. This antibody is expected to recognize both reported isoforms (NP_001003931.2, NP_001003935.2).

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human (100%), Gorilla (93%), Gibbon (87%), Bovine (87%), Horse (87%), Opossum (87%), Monkey (80%), Mouse (80%), Rat (80%), Hamster (80%), Elephant (80%), Bat (80%), Turkey (80%), Chicken (80%), Trout (80%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide YEDYNCTLNQTNIEN from an internal region of human PARP3 (NP_001003931.2, NP_001003935.2). Percent identity with other species by BLAST analysis: Human (100%), Gorilla (93%), Gibbon (87%), Bovine (87%), Horse (87%), Opossum (87%), Monkey (80%), Mouse (80%), Rat (80%), Hamster (80%), Elephant (80%), Bat (80%), Turkey (80%), Chicken (80%), Trout (80%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:2000)

Usage: Peptide ELISA: antibody detection limit dilution 1:2000. Western Blot: Preliminary experiments in lysates of cell lines Daudi, HeLa and HepG2 gave no specific signal but low background (at antibody concentration up to 1 μg/mL). We would appreciate any feedback from people in the field - have any results been reported with other antibodies / lysates?

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for PARP3

Target: PARP3 (Poly (ADP-Ribose) Polymerase Family, Member 3 (PARP3))

Alternative Name: PARP3 (PARP3 Products)

Synonyms: ADPRT-3 antibody, PARP-3 antibody, PM38 antibody, Adprtl3 antibody, fj17c06 antibody, wu:fj17c06 antibody, zgc:66157 antibody, PARP3 antibody, parp3 antibody, ADPRT3 antibody, ADPRTL2 antibody, ADPRTL3 antibody, ARTD3 antibody, IRT1 antibody, PADPRT-3 antibody, A930002C11Rik antibody, AW990611 antibody, Adprt3 antibody, pADPRT-3 antibody, seed maturation protein PM38 antibody, poly (ADP-ribose) polymerase family, member 3 antibody, poly(ADP-ribose) polymerase family member 3 antibody, poly(ADP-ribose) polymerase family member 3 L homeolog antibody, PARP3 antibody, parp3 antibody, parp3.L antibody, Parp3 antibody

Background: Name/Gene ID: PARP3

Synonyms: PARP3, ADPRT-3, ADPRTL2, ADPRTL3, ARTD3, ADPRT3, HPARP-3, PADPRT-3, Poly[ADP-ribose] synthase 3, Poly[ADP-ribose] synthetase 3, PARP-3, Poly [ADP-ribose] polymerase 3, IRT1, NAD+ ADP-ribosyltransferase 3, Poly(ADP-ribose) synthetase-3

Gene ID: 10039

NCBI Accession: NP_001003931, NP_001003935

UniProt: Q9Y6F1