RPA1 antibody

Catalog No. ABIN5964078

Product Details anti-RPA1 Antibody

Target: RPA1 (Replication Protein A1, 70kDa (RPA1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purification: Affinity purification

Immunogen: Recombinant fusion protein of human RPA1 (NP_002936.1).

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000 IHC 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for RPA1

Target: RPA1 (Replication Protein A1, 70kDa (RPA1))

Alternative Name: RPA1 (RPA1 Products)

Synonyms: HSSB antibody, MST075 antibody, REPA1 antibody, RF-A antibody, RP-A antibody, RPA70 antibody, Cb1-727 antibody, wu:fi14b08 antibody, zgc:55337 antibody, zgc:77092 antibody, RPA1 antibody, hssb antibody, mst075 antibody, repa1 antibody, rf-a antibody, rp-a antibody, rpa antibody, rpa70 antibody, LOC100230990 antibody, 5031405K23Rik antibody, 70kDa antibody, AA589576 antibody, AW557552 antibody, Rpa antibody, CG9633 antibody, D-RPA70 antibody, D-SSB antibody, DRP-A antibody, DmRPA antibody, Dmel\\CG9633 antibody, RPA antibody, RPA 70 antibody, RPA 70 kDa antibody, RpA70 antibody, Rpa-70 antibody, Ssb-70 antibody, dRP-A antibody, dmrpa1 antibody, i164 antibody, replication protein A1 antibody, replication protein A1, 70kDa antibody, replication protein A1 L homeolog antibody, Replication Protein A 70 antibody, RPA1 antibody, Rpa1 antibody, rpa1 antibody, rpa1.L antibody, RpA-70 antibody

Background: This gene encodes the largest subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The nucleoprotein complex protects the single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. This subunit contains four oligonucleotide/oligosaccharide-binding (OB) domains, though the majority of ssDNA binding occurs in two of these domains. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which ssDNA binding domains are utilized. The different binding modes differ in the length of DNA bound and in the proteins with which it interacts, thereby playing a role in regulating different genomic maintenance pathways.

Molecular Weight:

Observed_MW: 70kDa

Calculated_MW: 68kDa

Gene ID: 6117

UniProt: P27694

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA