ABCB1 antibody (AA 863-912)

Catalog No. ABIN6745748

Product Details anti-ABCB1 Antibody

Target: ABCB1 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1 (ABCB1))

Binding Specificity: AA 863-912

Reactivity: Human, Mouse, Rat, Rabbit, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ABCB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human ABCB1 / P Glycoprotein

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Rabbit (100%) Sheep, Dog, Bovine, Pig (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa863-912 of human ABCB1 (P08183, NP_000918). Percent identity by BLAST analysis: Human, Mouse, Rat, Hamster, Rabbit (100%), Gorilla, Gibbon, Monkey, Galago, Sheep, Panda, Dog, Cat, Bovine, Bat, Pig (92%), Marmoset, Horse (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1.0 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for ABCB1

Target: ABCB1 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1 (ABCB1))

Alternative Name: ABCB1 / MDR1 / P Glycoprotein (ABCB1 Products)

Synonyms: ABC20 antibody, CD243 antibody, CLCS antibody, GP170 antibody, MDR1 antibody, P-GP antibody, PGY1 antibody, Abcb1 antibody, Mdr1a antibody, p-gp antibody, xemdr antibody, Mdr1 antibody, Mdr1b antibody, Pgy-1 antibody, Pgy1 antibody, mdr antibody, ABCB1 antibody, PGP1 antibody, ATP binding cassette subfamily B member 1 antibody, ATP binding cassette subfamily B member 1A antibody, ATP binding cassette subfamily B member 1 L homeolog antibody, ATP-binding cassette, sub-family B (MDR/TAP), member 1B antibody, ABCB1 antibody, Abcb1a antibody, abcb1.L antibody, Abcb1b antibody, Abcb1 antibody

Background: Name/Gene ID: ABCB1
Subfamily: ATP-binding cassette - ABCB/MDR
Family: Transporter

Synonyms: ABCB1, ABC20, Abcb1b, CLCS, Colchicin sensitivity, CD243, Doxorubicin resistance, IBD13, gp170, MDR1, Multidrug resistance protein 1, P glycoprotein, P-glycoprotein 1, P-GP, CD243 antigen, PGY1

Gene ID: 5243

NCBI Accession: NP_000918

UniProt: P08183