MCAM antibody
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- Target See all MCAM Antibodies
- MCAM (Melanoma Cell Adhesion Molecule (MCAM))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This MCAM antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)
- Purpose
- Mouse Monoclonal anti-MUC18 / Mucin 18 / CD146 (MUC18/1130), Purified, BSA-free
- Characteristics
- The human Mel-CAM gene maps to chromosome 11q23 and encodes a trans-membrane glycoprotein, also designated MCAM, MUC 18 or CD146, that belongs to the immunoglobulin superfamily and functions as a Ca2 -independent cell adhesion molecule. Mel-CAM expression is restricted to advanced primary and metastatic melanomas and to cell lines of the neuroectodermal lineage, but not normal melanocytes. Mel-CAM is found on 80 % of advanced primary human melanomas and correlates well with development of metastatic disease. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Purification
- Purified
- Immunogen
- Recombinant human MUC18 protein
- Clone
- MUC18-1130
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product MCAM Primary Antibody
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- Application Notes
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Immunohistology (formalin)
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 0.5-1.0 μg/mL
- Optimal dilution for a specific application should be determined by user
- Comment
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A-375, HUV-EC or HeLa Cells. Tonsil or Melanoma.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS (no BSA, no azide)
- Preservative
- Azide free
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- Target
- MCAM (Melanoma Cell Adhesion Molecule (MCAM))
- Alternative Name
- MUC18 / Mucin 18 / CD146 (MCAM Products)
- Synonyms
- CD146 antibody, MUC18 antibody, Muc18 antibody, 1-gicerin antibody, AV025631 antibody, CD149 antibody, s-endo antibody, s-gicerin antibody, cd146 antibody, gicerin antibody, id:ibd3512 antibody, mcam antibody, MCAM antibody, melanoma cell adhesion molecule antibody, melanoma cell adhesion molecule b antibody, MCAM antibody, Mcam antibody, mcamb antibody
- Molecular Weight
- 130 kDa
- Gene ID
- 4162, 599039
- UniProt
- P43121
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