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FAT Atypical Cadherin 2 (FAT2) antibody (CF®647)

FAT2 Reactivity: Human IF, FACS, IHC Host: Mouse Monoclonal 8C5 CF®647
Catalog No. ABIN6155399
  • Target See all FAT Atypical Cadherin 2 (FAT2) Antibodies
    FAT Atypical Cadherin 2 (FAT2)
    Reactivity
    • 14
    • 1
    • 1
    Human
    Host
    • 9
    • 5
    Mouse
    Clonality
    • 9
    • 5
    Monoclonal
    Conjugate
    • 5
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    CF®647
    Application
    • 10
    • 9
    • 9
    • 3
    • 1
    • 1
    • 1
    • 1
    Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC)
    Purpose
    Mouse Monoclonal anti-Protocadherin-FAT2 (8C5), CF647 Conjugate
    Characteristics
    This antibody recognizes a protein of 480 kDa, which is identified as FAT2. The cadherins represent a family of Ca2 -dependent adhesion molecules that function to mediate cell-to-cell binding that is critical for the maintenance of structure and morphogenesis. Cadherins each contain a large extracellular domain at the N-terminus, which is characterized by a series of five homologous repeats, the most distal of which is thought to be responsible for binding specificity. The relatively short C-terminal intracellular domain interacts with a variety of cytoplasmic proteins, including ?-catenin, to regulate cadherin function. The cadherin superfamily includes cadherins, protocadherins, desmogleins and desmocollins. FAT2 (FAT tumor suppressor homolog 2) is a single-pass type I membrane protein that belongs to the protocadherin subfamily of cadherins. FAT2 contains one Laminin G-like domain, two EGF-like domains and 32 cadherin domains and is believed to function as a cell adhesion molecule, controlling cell proliferation and playing an important role in cerebellum development. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
    Immunogen
    Purified recombinant human FAT2 fusion protein
    Clone
    8C5
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product FAT2 Primary Antibody
  • Application Notes
    Immunohistology (formalin) 0.5-1 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Immunofluorescence 1-2 μg/mL
    • Optimal dilution for a specific application should be determined by user
    Comment

    Esophagus, Cervix, Uterus, Tonsil or Cerebellum.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    100 μg/mL
    Buffer
    PBS/0.1 % BSA/0.05 % azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Protect from light
  • Target
    FAT Atypical Cadherin 2 (FAT2)
    Alternative Name
    Protocadherin-FAT2 (FAT2 Products)
    Synonyms
    EMI2 antibody, Fath2 antibody, Gm523 antibody, mKIAA0811 antibody, CDHF8 antibody, CDHR9 antibody, HFAT2 antibody, MEGF1 antibody, FAT atypical cadherin 2 antibody, Fat2 antibody, FAT2 antibody
    Molecular Weight
    480 kDa
    Gene ID
    2196, 591255
    UniProt
    Q9NYQ8
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