DNASE1 antibody (AA 1-252)

Catalog No. ABIN615834

Product Details anti-DNASE1 Antibody

Target: DNASE1 (Deoxyribonuclease I (DNASE1))

Binding Specificity: AA 1-252

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DNASE1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody recognizes Deoxyribonuclease I (DNASE1).

Cross-Reactivity (Details): Species reactivity (expected):Rabbit (81 %), Dogs (81 %).
Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography

Immunogen: Recombinant protein fragment containing a sequence corresponding to a region within amino acids 1 and 252 of DNase I. Genename: DNASE1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: 0.1 M Tris-glycine, pH 7.0 containing 10 % Glycerol and 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for DNASE1

Target: DNASE1 (Deoxyribonuclease I (DNASE1))

Alternative Name: Deoxyribonuclease-1 (DNASE1 Products)

Synonyms: LOC397802 antibody, DNL1 antibody, DRNI antibody, AI788650 antibody, DNaseI antibody, Dnl1 antibody, dnase1-A antibody, zgc:92440 antibody, DNase I antibody, deoxyribonuclease 1 antibody, deoxyribonuclease I antibody, deoxyribonuclease I L homeolog antibody, Deoxyribonuclease I antibody, endA antibody, LOC397802 antibody, DNASE1 antibody, Dnase1 antibody, dnase1.L antibody, dnase1 antibody, CpipJ_CPIJ011184 antibody, CpipJ_CPIJ011185 antibody, CpipJ_CPIJ011186 antibody, CpipJ_CPIJ011187 antibody, CpipJ_CPIJ011190 antibody, CpipJ_CPIJ017803 antibody, Desal_1409 antibody, MPET_RS05255 antibody, METEV_RS02275 antibody, Plabr_0631 antibody

Background: Deoxyribonuclease I (EC 3.1.21.1) gene is approximately 3.2 kb long with 9 exons separated by 8 introns. In the form of a bovine pancreatic enzyme preparation, it occupies an important place in the history of protein chemistry and enzymology: it was the first enzyme to be recognized as specific for DNA, it was the first DNase to be crystallized, and it was the first DNase for which a specific protein inhibitor was characterized. DNase I is a Ca2+ and Mg2+ dependant endonuclease. DNase I is synthesized in the pancreas and stored in zymogen granules. It has been used to reduce the viscosity of cystic fibrosis sputum. A DNase I-like enzyme appears to catalyze the degradation of chromatin to oligo- and mononucleosomes during apoptosis. A recent study has demonstrated an endonuclease with activity and antigenicity indistinguishable from DNase I in thymocytes, cells susceptible to apoptosis. DNase I is an endonuclease that hydrolyzes double-stranded or single stranded DNA preferentially at sites adjacent to pyrimidine nucleotides. The product of hydrolysis is a complex mixture of 5'-phosphate mononucleotides and oligonucleotides. In the presence of Mg ion, DNase I attacks each strand of DNA independently and the cleavage sites are random.Synonyms: DNASE1, DNL1, DNase I, DRNI, Deoxyribonuclease I

Gene ID: 1773

NCBI Accession: NP_005214

UniProt: P24855