UACA antibody
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- Target See all UACA Antibodies
- UACA
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Reactivity
- Human, Mouse
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This UACA antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)
- Purpose
- Mouse Monoclonal anti-UACA / Nucling (AE-5), Purified, BSA-free
- Characteristics
- UACA (Uveal Autoantigen with Coiled-coil domains and Ankyrin repeats) is a 1,416 amino acid nuclear membrane protein. It was originally identified as an autoantigen in patients with panuveitis, a characteristic of Vogt-Koyanagi-Harada disease, and in patients with Graves' disease. UACA was also later identified as Nucling, an mRNA differentially expressed in F9 embryonal carcinoma cells during cardiac muscle differentiation. UACA appears to function as a pro-apoptotic protein that recruits the apaf-1-pro-caspase-9 complex for the induction of apoptosis to mediate the cell-death pathway. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Purification
- Purified
- Immunogen
- Nuclei of myeloid leukemia biopsy cells
- Clone
- AE-5
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product UACA Primary Antibody
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- Application Notes
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Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 0.5-1.0 μg/mL
- Western blotting 0.5-1 μg/mL
- Optimal dilution for a specific application should be determined by user
- Comment
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HeLa or 293T cells. Highly expressed in skeletal muscle, heart, kidney and pancreas. Also expressed in epidermal melanocytes, eye muscles and thyroid follicular cells.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS (no BSA, no azide)
- Preservative
- Azide free
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- Target
- UACA
- Alternative Name
- UACA / Nucling (UACA Products)
- Synonyms
- NUCLING antibody, 2700059D02Rik antibody, nucling antibody, RGD1560011 antibody, uveal autoantigen with coiled-coil domains and ankyrin repeats antibody, UACA antibody, Uaca antibody
- Background
- Nuclear membrane binding protein, Uveal autoantigen with coiled-coil domains and ankyrin repeats (UACA), NUCLING
- Molecular Weight
- 160 kDa
- Gene ID
- 55075, 108049
- UniProt
- Q9BZF9
- Pathways
- Positive Regulation of Endopeptidase Activity
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