CD45RB (CD45RB) antibody (APC)

Details for Product No. ABIN6170638
Antigen
  • B220
  • CD45
  • CD45R
  • GP180
  • L-CA
  • LCA
  • LY5
  • T200
  • Cd45
  • Ly-5
  • Lyt-4
  • loc
  • protein tyrosine phosphatase, receptor type C
  • protein tyrosine phosphatase, receptor type, C
  • PTPRC
  • Ptprc
Reactivity
Human
242
74
16
6
5
4
3
1
Host
Mouse
219
77
24
Clonality (Clone)
Monoclonal ()
Conjugate
APC
33
31
20
19
9
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
4
3
3
2
2
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
295
167
145
103
55
53
21
17
7
3
2
1
Options
Purpose Mouse Monoclonal anti-CD45RB (PTPRC/1132), APC Conjugate
Immunogen Recombinant full-length human CD45RB protein
Clone PTPRC-1132
Isotype IgG1, kappa
Characteristics CD45R, also designated CD45 and PTPRC, is identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45RB is expressed on mature B-lymphocytes and the majority of lymphomas and leukemias of B-cell origin. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Antigen
Alternative Name CD45RB (CD45RB Antibody Abstract)
Background B220, CD45R, GP180, Leukocyte common antigen (LCA), Loc, Ly-5, Lyt-4, Protein tyrosine phosphatase receptor type C (PTPRC), Receptor-type tyrosine-protein phosphatase C, T200 glycoprotein
Molecular Weight 180-220 kDa
Gene ID 5788, 654514
UniProt P08575
Application Notes Does not react with rat or monkey, others not known
  • Immunohistology (formalin)
  • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
  • Immunofluorescence 0.5-1 μg/mL
  • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
  • Western blotting 0.5-1.0 μg/mL
  • Optimal dilution for a specific application should be determined by user
Comment

Ramos, U-698, or GA-10 cells. Tonsil

Restrictions For Research Use only
Format Liquid
Concentration 100 μg/mL
Buffer PBS/0.1 % BSA/0.05 % azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Supplier Images
Image no. 1 for anti-CD45RB (CD45RB) antibody (APC) (ABIN6170638) anti-CD45RB (CD45RB) antibody (APC) (Image 1)
Image no. 2 for anti-CD45RB (CD45RB) antibody (APC) (ABIN6170638) anti-CD45RB (CD45RB) antibody (APC) (Image 2)
Image no. 3 for anti-CD45RB (CD45RB) antibody (APC) (ABIN6170638) anti-CD45RB (CD45RB) antibody (APC) (Image 3)