TGFA antibody (C-Term) (CF®405S)

Catalog No. ABIN6171373

Product Details anti-TGFA Antibody (CF®405S)

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Binding Specificity: C-Term

Reactivity: Human, Rat, Mouse, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This TGFA antibody is conjugated to CF®405S

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Purpose: Mouse Monoclonal anti-TGF alpha (MF9), CF405S Conjugate

Characteristics: This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with Ab-1 is completely blocked by the peptide used for raising this antibody. TGF? ? (aa50) is a growth factor with 33 % homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: A 17-amino acid synthetic peptide from the carboxyl-terminus of rat TGF?.

Clone: MF9

Isotype: IgG1 kappa

Application Details

Application Notes: Immunohistology formalin-fixed 2-4 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

Jurkat or Ramos cells. Testicular Carcinoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for TGFA

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Alternative Name: TGF alpha (TGFA Products)

Synonyms: TFGA antibody, LOC100009482 antibody, TGF alpha antibody, TGFA antibody, wa-1 antibody, wa1 antibody, RATTGFAA antibody, TGFAA antibody, NV15606 antibody, si:ch211-10e8.7 antibody, transforming growth factor alpha antibody, transforming growth factor alpha L homeolog antibody, transforming growth factor-alpha antibody, transforming growth factor, alpha antibody, TGFA antibody, TGF-A antibody, Tgfa antibody, tgfa.L antibody, LOC100121300 antibody, LOC100009150 antibody, tgfa antibody

Background: EGF-like TGF, ETGF, TFGA, TGF Type 1, TGFA, Wa1, Waved 1

Molecular Weight: 6 kDa

Gene ID: 7039, 170009

UniProt: P01135

Pathways: NF-kappaB Signaling, RTK Signaling, EGFR Signaling Pathway