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KRT14 antibody (Keratin 14) (CF®660R) Primary Antibody

KRT14 Reactivity: Human, Mouse, Rat FACS, IF, IHC (f) Host: Mouse Monoclonal KRT14-532 CF®660R
Catalog No. ABIN6172524
$270.77
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  • Target See all KRT14 Antibodies
    KRT14
    Reactivity
    • 102
    • 62
    • 59
    • 11
    • 7
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rat
    Host
    • 57
    • 49
    • 3
    Mouse
    Clonality
    • 59
    • 50
    Monoclonal
    Conjugate
    • 69
    • 6
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This KRT14 antibody is conjugated to CF®660R
    Application
    • 56
    • 41
    • 35
    • 31
    • 25
    • 24
    • 18
    • 14
    • 10
    • 6
    • 4
    • 4
    • 3
    • 3
    • 1
    • 1
    Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    Purpose
    Mouse Monoclonal anti-Cytokeratin 14 (KRT14/532), CF660R Conjugate
    Characteristics
    Cytokeratin 14 (CK14) belongs to the type I (or A or acidic) subfamily of low molecular weight keratins and exists in combination with keratin 5 (type II or B or basic). CK14 is found in basal cells of squamous epithelia, some glandular epithelia, myoepithelium, and mesothelial cells. Anti-CK14 is useful in differentiating squamous cell carcinomas from poorly differentiated epithelial tumors. Anti-CK14 is one of the specific basal markers for distinguishing between basal and non-basal subtypes of breast carcinomas. Anti-CK14 is also a good marker for differentiation of intraductal from invasive salivary duct carcinoma by the positive staining of basal cells surrounding the in-situ neoplasm as well as for differentiation of benign prostate from prostate carcinoma. Furthermore, this antibody has been useful in separating oncocytic tumors of the kidney from its renal mimics, and in identifying metaplastic carcinomas of the breast. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
    Immunogen
    Recombinant full-length human KRT14 protein
    Clone
    KRT14-532
    Isotype
    IgG3
  • Application Notes
    Immunohistology formalin-fixed 0.5-1.0 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Immunofluorescence 0.5-1 μg/mL
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Optimal dilution for a specific application should be determined by user
    Comment

    A431 cells. Skin, Tonsil, Pro

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    100 μg/mL
    Buffer
    PBS/0.1 % BSA/0.05 % azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Protect from light
  • Target
    KRT14
    Alternative Name
    Cytokeratin 14 (KRT14 Products)
    Synonyms
    CK14, EBS3, EBS4, K14, NFJ, AI626930, Krt-1.14, Krt1-14, Ka14, ouro2, keratin 14, keratin 14, type I L homeolog, KRT14, Krt14, krt14.L
    Background
    CK-14, Dowling Meara, ebs3, ebs4, Epidermolysis Bullosa Simplex, k14, Keratin 14, Keratin Type I Cytoskeletal 14, Koebner, krt14, NFJ
    Molecular Weight
    ~50 kDa
    Gene ID
    3861, 6545380
    UniProt
    P02533
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