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FLI1 antibody (Friend Leukemia Virus Integration 1) (AA 107-261) (CF®770) Primary Antibody

FLI1 Reactivity: Human FACS, IF, WB Host: Mouse Monoclonal FLI1-1312 CF®770
Catalog No. ABIN6172642
$270.77
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  • Target See all FLI1 Antibodies
    FLI1
    Binding Specificity
    • 15
    • 12
    • 7
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 107-261
    Reactivity
    • 79
    • 27
    • 21
    • 9
    • 7
    • 6
    • 6
    • 6
    • 5
    • 5
    • 5
    • 4
    • 4
    • 3
    Human
    Host
    • 51
    • 26
    • 2
    Mouse
    Clonality
    • 53
    • 26
    Monoclonal
    Conjugate
    • 52
    • 5
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This FLI1 antibody is conjugated to CF®770
    Application
    • 70
    • 31
    • 30
    • 21
    • 19
    • 8
    • 7
    • 6
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)
    Purpose
    Mouse Monoclonal anti-FLI1 (FLI1/1312), CF770 Conjugate
    Characteristics
    This antibody recognizes a protein of 51 kDa, which is identified as FLI1. This protein, a member of the ETS family of DNA binding transcription factors, is involved in cellular proliferation and tumorigenesis. Ets-1 is the prototype member of a family of genes identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus.Members of the Ets gene family share a highly conserved carboxy-terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence. Approximately 90 % of Ewing s Sarcoma (EWS)/Primitive Neuroectodermal Tumors (PNET) have a specific translocation, t(11,22)(q24,q12), which results in fusion of EWS to Fli-1, and production of an EWS-Fli-1 fusion protein. Among normal tissues only endothelial cells and small lymphocytes express Fli-1. This protein is expressed in majority of vascular tumors including angiosarcomas, hemangioendotheliomas, hemangiomas, and Kaposi s Sarcomas. High sensitivity and specificity of Fli-1 equals to or exceeds that of the established vascular markers like CD31, CD34, and Factor VIII. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
    Immunogen
    Recombinant fragment (aa 107-261) of human FLI1 protein (exact sequence is proprietary)
    Clone
    FLI1-1312
    Isotype
    IgG
  • Application Notes
    Western blotting 1-2 μg/mL
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Immunofluorescence 1-2 μg/mL
    • Optimal dilution for a specific application should be determined by user
    Comment

    THP1 and Raji cells. Tonsil, Ewing s sarcoma (EWS) and Angiosarcoma

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    100 μg/mL
    Buffer
    PBS/0.1 % BSA/0.05 % azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Protect from light
  • Target
    FLI1
    Alternative Name
    FLI1 (FLI1 Products)
    Synonyms
    EWSR2, SIC-1, FLI1, fli, Xfli, fli1, Xfli1, fli-1, Xfli-1, MGC121593, Fli-1, Sic1, cb855, wu:fc45b11, xlfli, Fli-1 proto-oncogene, ETS transcription factor, Fli-1 proto-oncogene, ETS transcription factor S homeolog, Friend leukemia integration 1, Fli-1 proto-oncogene, ETS transcription factor a, Fli-1 proto-oncogene, ETS transcription factor L homeolog, FLI1, Fli1, fli1.S, fli1, fli1a, fli1.L
    Molecular Weight
    51 kDa
    Gene ID
    2313, 504281
    UniProt
    Q01543
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